Zhang Feng-Xia, Sun Qin-Jian, Zheng Xing-Yue, Lin You-Ting, Shang Wei, Wang Ai-Hua, Duan Rui-Sheng, Chi Zhao-Fu
Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, No. 324 jingwuweiqi Road, Jinan, 250021, China.
Cell Mol Neurobiol. 2014 Aug;34(6):813-24. doi: 10.1007/s10571-014-0068-3. Epub 2014 May 16.
Temporal lobe epilepsy is characterized by spontaneous recurrent seizures (SRS) and associated with behavioral problems. However, the molecular mechanisms underlying these problems are not yet clear. In this study, kainic acid (KA) was systemically administered to immature male Wistar rats to induce SRS. The behavior of the immature rats was evaluated with a water maze, elevated-plus mazes, and open field tests. The expression patterns of synaptophysin, SNAP-25, and synaptotagmin 1 (Syt 1) were examined by reverse-transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis. KA-treated rats with SRS demonstrated learning and memory deficits, reduced anxiety, and increased locomotor activity, compared with placebo-treated rats and KA-treated rats without SRS. No neuronal cell loss was observed in the hippocampus 6 weeks after exposure to KA. However, RT-PCR and Western blot analyses revealed decreased synaptophysin, SNAP-25, and Syt 1 expression in KA-treated rats with SRS. Synaptophysin, SNAP-25, and Syt1 expression levels were found to be positively correlated with learning and memory but negatively correlated with anxiety and locomotor activity. These data suggested that SRS may induce changes in synaptophysin, SNAP-25, and Syt1 expression and may be functionally related to SRS-induced behavioral deficits.
颞叶癫痫的特征是自发性反复癫痫发作（SRS），并伴有行为问题。然而，这些问题背后的分子机制尚不清楚。在本研究中，将 kainic 酸（KA）全身注射给未成熟雄性 Wistar 大鼠以诱导 SRS。通过水迷宫、高架十字迷宫和旷场试验评估未成熟大鼠的行为。通过逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析检测突触素、SNAP-25 和突触结合蛋白 1（Syt 1）的表达模式。与安慰剂处理的大鼠和未发生 SRS 的 KA 处理大鼠相比，发生 SRS 的 KA 处理大鼠表现出学习和记忆缺陷、焦虑减轻和运动活动增加。暴露于 KA 后 6 周，在海马体中未观察到神经元细胞丢失。然而，RT-PCR 和蛋白质免疫印迹分析显示，发生 SRS 的 KA 处理大鼠的突触素、SNAP-25 和 Syt 1 表达降低。发现突触素、SNAP-25 和 Syt1 的表达水平与学习和记忆呈正相关，但与焦虑和运动活动呈负相关。这些数据表明，SRS 可能诱导突触素、SNAP-25 和 Syt1 的表达变化，并且可能在功能上与 SRS 诱导的行为缺陷相关。
