Wang Xiaoqing, Tian Shuang, Qu Zhenzhen, Meng Ran, Ni Guangxiao, Liu Min, Cao Huifang
The Department of Rehabilitation, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.
The Department of Neurology, Shijiazhuang People's Hospital, Shijiazhuang, Hebei, People's Republic of China.
Sci Rep. 2025 Apr 23;15(1):14142. doi: 10.1038/s41598-025-99259-3.
The objective of this study was to explore the protective effects of hydroxy fullerenes (HFs) on cognitive function in rats with temporal lobe epilepsy (TLE) and to elucidate the underlying mechanisms. Eighteen Sprague-Dawley (SD) rats were randomly selected and administered pilocarpine (50 mg/kg) intraperitoneally to establish a TLE model, and were then randomly assigned to the TLE group and the TLE + HFs group. An additional nine SD rats were served as a normal control group (CON group). The Morris water maze (MWM) test was utilized to assess the spatial learning and memory capabilities of the rats. Nissl staining was employed to observe the survival neurons in the CA1 and CA3 regions. In addition, the ultrastructure of synapses in the CA1 region was examined using transmission electron microscopy (TEM). The expressions of postsynaptic densitin-95 (PSD-95) and synaptophysin (SYP) in the hippocampus were detected via western blotting. The findings revealed that compared to the CON group, the TLE group exhibited significantly prolonged escape latency, reduced platform crossing frequency, and shortened time spent in the target quadrant. The number of surviving neurons in the CA1 and CA3 regions and the expression of PSD95 and SYP protein were significantly decreased (P < 0.05 or P < 0.001). However, these alterations were reversed in the TLE + HFs group. It is suggested that HFs may enhance the spatial learning and memory ability of TLE rats by preserving the integrity of hippocampal neurons, up-regulating the expression of SYP and PSD95 in hippocampus.
本研究的目的是探讨羟基富勒烯（HFs）对颞叶癫痫（TLE）大鼠认知功能的保护作用，并阐明其潜在机制。随机选取18只Sprague-Dawley（SD）大鼠，腹腔注射匹罗卡品（50 mg/kg）建立TLE模型，然后随机分为TLE组和TLE + HFs组。另外9只SD大鼠作为正常对照组（CON组）。采用Morris水迷宫（MWM）试验评估大鼠的空间学习和记忆能力。采用尼氏染色观察CA1和CA3区存活神经元。此外，使用透射电子显微镜（TEM）检查CA1区突触的超微结构。通过蛋白质免疫印迹法检测海马中突触后致密蛋白95（PSD-95）和突触素（SYP）的表达。结果显示，与CON组相比，TLE组逃避潜伏期显著延长，平台穿越频率降低，在目标象限停留时间缩短。CA1和CA3区存活神经元数量以及PSD95和SYP蛋白表达显著降低（P < 0.05或P < 0.001）。然而，这些改变在TLE + HFs组中得到逆转。提示HFs可能通过保持海马神经元的完整性、上调海马中SYP和PSD95的表达来增强TLE大鼠的空间学习和记忆能力。
