Department of Geriatrics, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shanxi, 710038, China.
Department of General Surgery, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shanxi, 710038, China.
Int J Biol Macromol. 2014 Aug;69:1-4. doi: 10.1016/j.ijbiomac.2014.05.014. Epub 2014 May 12.
A neutral polysaccharide fraction (CGPP) was extracted from Gynostemma pentaphyllum by water extraction and ethanol precipitation. Gas chromatography (GC) analysis showed that the CGPP was mainly composed of mannose, glucose, arabinose, rhamnose, galactose and glucuronic acid in molar ratios of 2.0:2.2:1.3:2.2:1.2:2.5. The present study aimed at evaluating the antitumor potentials of CGPP on the growth of H22 tumor transplanted in mice and the underlying mechanism. The results showed that CGPP (50 and 200mg/kg) could effectively inhibit the solid tumor growth of H22 hepatocarcinoma transplanted in ICR mice. Besides, the body weight, spleen/thymus indexes and splenocytes proliferation of H22 tumor bearing mice were also improved in CGPP-treated groups. Furthermore, the level of the cytokines, such as IL-2, TNF-α and IFN-γ, as well as the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTL) in tumor-bearing mice were markedly promoted by CGPP oral administration. In addition, CGPP treatment greatly prolonged the survival period in H22 ascites tumor-bearing mice. Taken together, these findings indicate that CGPP has antitumor activity in vivo at least partly via improving immune responses of host organism, and seems to be a safe and effective supplementary agent for the treatment of hepatocellular carcinoma (HCC).
从绞股蓝中提取出一种中性多糖（CGPP），采用水提醇沉法。气相色谱（GC）分析表明，CGPP 主要由甘露糖、葡萄糖、阿拉伯糖、鼠李糖、半乳糖和葡萄糖醛酸以摩尔比 2.0：2.2：1.3：2.2：1.2：2.5 组成。本研究旨在评估 CGPP 对 H22 荷瘤小鼠肿瘤生长的抗肿瘤潜力及其作用机制。结果表明，CGPP（50 和 200mg/kg）可有效抑制 ICR 小鼠移植的 H22 肝癌实体瘤的生长。此外，CGPP 治疗组荷瘤小鼠的体重、脾/胸腺指数和脾细胞增殖也得到改善。此外，CGPP 口服给药还显著提高了荷瘤小鼠细胞因子（如 IL-2、TNF-α 和 IFN-γ）的水平以及自然杀伤（NK）细胞和细胞毒性 T 淋巴细胞（CTL）的活性。此外，CGPP 治疗大大延长了 H22 腹水瘤荷瘤小鼠的存活期。综上所述，这些发现表明 CGPP 具有体内抗肿瘤活性，至少部分是通过改善宿主的免疫反应，并且似乎是治疗肝细胞癌（HCC）的一种安全有效的辅助药物。
