Schepens Eye Research Institute and Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts, United States.
JG Machine Company, Inc., Wilmington, Massachusetts, United States.
Invest Ophthalmol Vis Sci. 2014 May 15;55(6):3681-5. doi: 10.1167/iovs.14-14058.
To establish a murine model for keratoprosthesis.
A miniature keratoprosthesis (m-KPro) device was created consisting of a poly[methyl methacrylate] front part and a titanium back plate, designed after the Boston KPro, which is in widespread clinical use. BALB/c mice were used and a 2 mm in diameter donor cornea was punched out. After 2-mm trepanation of the syngeneic recipient cornea, extracapsular crystalline lens extraction was performed. The m-KPro was assembled onto the cornea button in a similar manner to human KPro implantation. The cornea-device complex was secured to the recipient bed with eight interrupted 11-0 sutures. All mice (n = 10) were followed up for 8 weeks postoperatively.
All m-KPros were successfully implanted and retained in all 10 animals. There were no critical complications such as endophthalmitis, corneal melting, device extrusions, leakage, extensive inflammation, or weight loss in the animals. We observed mild to moderate donor and host corneal neovascularization in all cases throughout the follow-up period.
We have established a novel murine model of KPro implantation that we anticipate will serve as a good experimental system for evaluating host responses after KPro surgery.
建立角膜假体的小鼠模型。
制作了一种微型角膜假体（m-KPro）装置，由聚甲基丙烯酸甲酯前体和钛背板组成，设计灵感源自广泛应用于临床的波士顿角膜假体。使用 BALB/c 小鼠，并从供体角膜上切取 2mm 直径的角膜瓣。在同系受者角膜上进行 2mm 环钻后，进行囊外晶状体切除术。以类似于人类角膜假体植入的方式，将 m-KPro 组装到角膜瓣上。用 8 根间断 11-0 缝线将角膜-假体复合物固定在受者床位上。所有小鼠（n=10）均在术后 8 周内进行随访。
所有 m-KPro 均成功植入并保留在 10 只动物体内。在动物中没有出现严重并发症，如眼内炎、角膜溶解、器械脱出、渗漏、广泛炎症或体重减轻。在整个随访期间，我们观察到所有病例均有轻度至中度供体和宿主角膜新生血管形成。
我们建立了一种新型的角膜假体植入小鼠模型，我们预计它将成为评估角膜假体手术后宿主反应的良好实验系统。
