Singh H, Herndon D N
Department of Surgery, University of Texas Medical Branch, Galveston 77550.
Immunopharmacol Immunotoxicol. 1989;11(4):631-44. doi: 10.3109/08923978909005391.
The effect of in vivo administration of Isoprinosine (ISO) on, i) the proliferation of splenic lymphocytes in response to the T-cell mitogen, concanavalin-A (Con-A) and, ii) the natural killer (NK) cell cytotoxicity was studied following a full skin thickness burn injury in a rat model. Administration of ISO (100 mg/kg body wt/day) twice daily, resulted in significant augmentation of the proliferative responses of lymphocytes compared to non-treated burned animals, at 7 days post injury. However, it did not effect the lymphoproliferation at 14 days post injury, the time period at which a complete suppression of lymphocyte proliferation was observed in burned non-treated animals. Also, the proliferation of lymphocytes from normal nonburned animals was not affected by treatment with ISO. ISO treatment of the burned animals resulted in a significant increase in the NK cytotoxicity compared to non-treated burned animals. As with Con-A responses, ISO administered to control nonburned animals did not have any effect on NK cell cytotoxicity. Our studies thus indicate that ISO can be a potential immunomodulator of suppressed immune function following thermal injury, particularly in patients whose lymphocyte responses to T cell mitogen Con-A are not completely suppressed.
在大鼠模型的全层皮肤烧伤损伤后，研究了体内给予异丙肌苷（ISO）对以下方面的影响：i）脾淋巴细胞对T细胞有丝分裂原刀豆球蛋白A（Con-A）的增殖反应，以及ii）自然杀伤（NK）细胞的细胞毒性。每天两次给予ISO（100 mg/kg体重/天），与未治疗的烧伤动物相比，在损伤后7天，淋巴细胞的增殖反应显著增强。然而，在损伤后14天，它对淋巴细胞增殖没有影响，而在未治疗的烧伤动物中，此时观察到淋巴细胞增殖被完全抑制。此外，正常未烧伤动物的淋巴细胞增殖不受ISO治疗的影响。与未治疗的烧伤动物相比，ISO治疗烧伤动物导致NK细胞毒性显著增加。与Con-A反应一样，给予对照未烧伤动物ISO对NK细胞毒性没有任何影响。因此，我们的研究表明，ISO可能是热损伤后免疫功能抑制的潜在免疫调节剂，特别是对于那些淋巴细胞对T细胞有丝分裂原Con-A的反应未被完全抑制的患者。
