Nemmani Kumar V S, Jena G B, Dey C S, Kaul C L, Ramarao P
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S. A. S. Nagar 160 062, India.
Indian J Exp Biol. 2002 Mar;40(3):282-7.
Immunomodulatory activity of an Ayurvedic polyherbal formulation, Immu-21 containing extracts of Ocimum sanctum, Withania somnifera, Emblica officinalis and Tinospora cordifolia was studied on proliferative response of splenic leukocytes to T cell mitogens, concanavalin (Con)-A and phytohemagglutinin (PHA) and B cell mitogen, lipopolysaccharide (LPS) in vitro by [3H]-thymidine uptake assay in mice. The cytotoxic activity of Immu-21 was tested by measuring the splenic leukocyte natural killer (NK) cell activity against K 562 cells. Intraperitoneal (i.p.) treatment with Immu-21 (30 mg/kg) once a day for 14 and 21 days did not cause change in body weight and spleen weight, where as splenocytes/spleen count was increased. Treatment of Immu-21 (30 mg/kg, i.p.) for 14 days and 1 mg/kg for 21 days significantly increased LPS induced leukocyte proliferation. NK cell activity was significantly increased when mice were pretreated with Immu-21 (10 and 30 mg/kg, i.p.) once a day for 7 days. The results indicate that pretreatment with Immu-21 selectively increased the proliferation of splenic leukocyte to B cell mitogen, LPS and cytotoxic activity against K 562 cells in mice.
研究了一种阿育吠陀多草药配方Immu - 21（含有神圣罗勒、印度人参、余甘子和心叶地锦提取物）对小鼠脾细胞体外对T细胞有丝分裂原刀豆蛋白A（Con - A）和植物血凝素（PHA）以及B细胞有丝分裂原脂多糖（LPS）增殖反应的免疫调节活性，采用[³H] - 胸腺嘧啶核苷摄取试验进行检测。通过测量脾细胞自然杀伤（NK）细胞对K 562细胞的活性来测试Immu - 21的细胞毒性活性。腹腔注射（i.p.）Immu - 21（30 mg/kg），每天一次，持续14天和21天，未引起体重和脾脏重量变化，但脾细胞/脾脏计数增加。Immu - 21（30 mg/kg，i.p.）处理14天和1 mg/kg处理21天显著增加了LPS诱导的白细胞增殖。当小鼠腹腔注射Immu - 21（10和30 mg/kg）预处理7天，每天一次时，NK细胞活性显著增加。结果表明，Immu - 21预处理可选择性增加小鼠脾细胞对B细胞有丝分裂原LPS的增殖以及对K 562细胞的细胞毒性活性。
