通过实时聚合酶链反应检测腺样体组织中的幽门螺杆菌。
Detection of Helicobacter pylori in adenoid tissue by real-time polymerase chain reaction.
作者信息
Kaymakçı Mustafa, Aydın Mehtap, Yazıcı Selçuk, Sağır Ozlem, Gür Ozer Erdem, Sayan Murat
机构信息
Department of Otolaryngology, Medical Faculty of Balıkesir University, 35100 Balıkesir, Turkey.
出版信息
Kulak Burun Bogaz Ihtis Derg. 2014 Mar-Apr;24(2):78-82. doi: 10.5606/kbbihtisas.2014.28445.
OBJECTIVES
This study aims to investigate the presence of Helicobacter pylori (H. pylori) using the real-time polymerase chain reaction (PCR) method in the adenoid tissues in children undergoing surgical operation due to adenoid hypertrophy.
PATIENTS AND METHODS
Adenoid tissues of 23 children (8 girls, 15 boys; mean age 6.2 years; range 3 to 9 years) who were operated with the diagnosis of adenoid hypertrophy, tonsil + adenoid hypertrophy or tonsil + adenoid hypertrophy + serous otitis media in our clinic between January 2012 and April 2012 were examined.
RESULTS
Of 23 patients, H. pylori was detected in the adenoid tissues of two (8.7%). Regurgitation was present in seven patients. However, no regurgitation was found in H. pylori-positive patients.
CONCLUSION
In this study the presence of H. pylori in patients with adenoid hypertrophy has been demonstrated using PCR method. To be able to support the hypothesis that H. pylori has a place in etiology of adenoid hypertrophy, multicenter studies are warranted.
目的
本研究旨在采用实时聚合酶链反应（PCR）方法，调查因腺样体肥大接受手术的儿童腺样体组织中幽门螺杆菌（H. pylori）的存在情况。
患者与方法
对2012年1月至2012年4月期间在我院因腺样体肥大、扁桃体 + 腺样体肥大或扁桃体 + 腺样体肥大 + 浆液性中耳炎诊断而接受手术的23名儿童（8名女孩，15名男孩；平均年龄6.2岁；范围3至9岁）的腺样体组织进行检查。
结果
23例患者中，2例（8.7%）腺样体组织检测到幽门螺杆菌。7例患者存在反流。然而，幽门螺杆菌阳性患者中未发现反流。
结论
本研究采用PCR方法证实了腺样体肥大患者中存在幽门螺杆菌。为了能够支持幽门螺杆菌在腺样体肥大病因学中占有一席之地这一假说，有必要开展多中心研究。
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