Department of Clinical Laboratory Medicine, Institute of Surgery Research, Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
Department of Neurology, Institute of Surgery Research, Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
Biosens Bioelectron. 2014 Oct 15;60:318-24. doi: 10.1016/j.bios.2014.04.027. Epub 2014 Apr 30.
A label-free and high-sensitive sensing technology for tumor cell recognition and detection was developed based on a novel 2 × 3 model of leaky surface acoustic wave (LSAW) aptasensor array. In this methodology, every resonator crystal unit of the LSAW aptasensor array had an individual oscillator circuit to work without mutual interference, and could oscillate independently with the phase shift stability of ± 0.15° in air phase and ± 0.3° in liquid phase. The aptamer was firstly assembled to the gold electrode surface of 100 MHz LiTaO3 piezoelectric crystal, which could effectively captured target cells (MCF-7 cells) based on the specific interaction between aptamer and the overexpression of MUC1 protein on tumor cell surface. The aptamer-cell complexes increased the mass loading of LSAW aptasensor and led to phase shifts of LSAW. The plot of phase shift against the logarithm of concentration of MCF-7 cells was linear over the range from 1 × 10(2) cells mL(-1) to 1 × 10(7) cells mL(-1) with a correlation coefficient of 0.994. The detection limit as low as 32 cells mL(-1) was achieved for MCF-7 cells. The LSAW aptasensor also exhibited excellent specificity and stability. In addition, this aptasensor could be regenerated for ten times without irreversible loss of activity. Therefore, the LSAW aptasensor may offer a promising approach for tumor cell detection and have great potential in clinical applications.
基于新型的 2×3 漏声表面波（LSAW）适配体传感器阵列，开发了一种用于肿瘤细胞识别和检测的无标记和高灵敏度传感技术。在这种方法中，LSAW 适配体传感器阵列的每个谐振器晶体单元都有一个独立的振荡器电路，可以在没有相互干扰的情况下工作，并能以空气相±0.15°和液相±0.3°的相位稳定度独立振荡。适配体首先被组装到 100 MHz LiTaO3 压电晶体的金电极表面，它可以基于适配体与肿瘤细胞表面过度表达的 MUC1 蛋白之间的特异性相互作用，有效地捕获靶细胞（MCF-7 细胞）。适配体-细胞复合物增加了 LSAW 适配体传感器的质量负载，导致 LSAW 的相移。相移与 MCF-7 细胞浓度的对数之间的关系呈线性，范围从 1×10(2)细胞 mL(-1)到 1×10(7)细胞 mL(-1)，相关系数为 0.994。MCF-7 细胞的检测限低至 32 个细胞 mL(-1)。LSAW 适配体传感器还表现出优异的特异性和稳定性。此外，这种适配体传感器可以再生十次而不会失去活性。因此，LSAW 适配体传感器可能为肿瘤细胞检测提供一种有前途的方法，并且在临床应用中具有很大的潜力。
