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菊花病毒B:哺乳动物中针对结构蛋白的抗体及Mg2(+)依赖性逆转录酶活性

Chrysanthemum virus B: antibodies to structural protein in mammals and Mg2(+)-dependent reverse-transcriptase activity.

作者信息

Morozov V A, Ilyinskii P O, Ilyin K V

机构信息

All-Union Oncological Centre, U.S.S.R. Academy of Medical Sciences, Moscow.

出版信息

Acta Virol. 1989 Dec;33(6):527-34.

PMID:2483603
Abstract

Human plasma has been found to contain antibodies reacting with the structural protein of chrysanthemum virus B (CV-B) and about 10 times less intensively with the structural protein of another carlavirus, the potato virus M. It has been shown that the antibodies bind to CV-B through their F(ab)2 fragments. No reaction with proteins of other plant viruses or retroviruses was observed. Antibodies reacting with CV-B protein are also present in the plasma of green monkey, goat, rabbit, and mouse, their level being somewhat lower than in man. In addition, Mg2(+)-dependent reverse transcriptase activity reaching maximum at 37 degrees C was detected in the CV-B preparations. It is suggested that humans and the mammals in question developed antibodies to CV-B which could enter into some enzymatic reactions at the body temperature.

摘要

已发现人血浆中含有与菊花病毒B(CV - B)结构蛋白发生反应的抗体,与另一种香石竹潜隐病毒——马铃薯病毒M的结构蛋白反应强度约低10倍。研究表明,这些抗体通过其F(ab)2片段与CV - B结合。未观察到与其他植物病毒或逆转录病毒蛋白的反应。与CV - B蛋白发生反应的抗体也存在于绿猴、山羊、兔子和小鼠的血浆中,其水平略低于人类。此外,在CV - B制剂中检测到在37℃达到最大值的Mg2(+)依赖性逆转录酶活性。有人提出,人类和上述哺乳动物产生了针对CV - B的抗体,这些抗体可能在体温下参与某些酶促反应。

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