Morozov V A, Il'inskiĭ P O, Il'in K V
Vopr Virusol. 1987 Sep-Oct;32(5):600-4.
Electrophoretic analysis of the protein composition of chrysanthemum B virus (ChrBV), a member of the carlavirus family, performed in 7.5-15% gradient polyacrylamide gel with 0.1% SDS revealed the presence in the preparation of two proteins with molecular weights of 34,000 and 37,000. The densitometric treatment showed their ratio approximating 2:1. Antibodies to these proteins were detected in a pool of normal human plasma and in individual human sera by the immune blotting test. No antibodies to ChrBV proteins were found in goat and rabbit sera. Normal human plasma contained no antibodies to structural proteins of tobacco mosaic, cucumber mosaic, and rock-cress mosaic viruses. It is presumed that the presence of antibody to ChrBV proteins may be associated either with casual structural relationship of viral and some cellular (serum) proteins or with immunization of man with ChrBV or immunologically related carlavirus(es).
在含有0.1%十二烷基硫酸钠（SDS）的7.5%-15%梯度聚丙烯酰胺凝胶中，对香石竹潜隐病毒属成员菊花B病毒（ChrBV）的蛋白质组成进行电泳分析，结果显示该制剂中存在分子量分别为34,000和37,000的两种蛋白质。光密度测定结果表明，它们的比例约为2:1。通过免疫印迹试验在正常人血浆池和个体人血清中检测到了针对这些蛋白质的抗体。在山羊和兔血清中未发现针对ChrBV蛋白质的抗体。正常人血浆中不存在针对烟草花叶病毒、黄瓜花叶病毒和芝麻菜花叶病毒结构蛋白的抗体。据推测，针对ChrBV蛋白质的抗体的存在可能与病毒和某些细胞（血清）蛋白质偶然的结构关系有关，或者与人感染ChrBV或免疫相关的香石竹潜隐病毒有关。
