[Antibodies to the structural proteins of the chrysanthemum B-virus in normal human plasma].

Electrophoretic analysis of the protein composition of chrysanthemum B virus (ChrBV), a member of the carlavirus family, performed in 7.5-15% gradient polyacrylamide gel with 0.1% SDS revealed the presence in the preparation of two proteins with molecular weights of 34,000 and 37,000. The densitometric treatment showed their ratio approximating 2:1. Antibodies to these proteins were detected in a pool of normal human plasma and in individual human sera by the immune blotting test. No antibodies to ChrBV proteins were found in goat and rabbit sera. Normal human plasma contained no antibodies to structural proteins of tobacco mosaic, cucumber mosaic, and rock-cress mosaic viruses. It is presumed that the presence of antibody to ChrBV proteins may be associated either with casual structural relationship of viral and some cellular (serum) proteins or with immunization of man with ChrBV or immunologically related carlavirus(es).