Parcell B J, Phillips G
Medical Microbiology, Ninewells Hospital and Medical School, NHS Tayside, Dundee, UK.
Medical Microbiology, Ninewells Hospital and Medical School, NHS Tayside, Dundee, UK.
J Hosp Infect. 2014 Jun;87(2):119-21. doi: 10.1016/j.jhin.2014.04.002. Epub 2014 Apr 18.
A meticillin-resistant Staphylococcus aureus (MRSA) polymerase chain reaction assay (Xpert(®) MRSA, Cepheid) was assessed for point-of-care testing (POCT) used by healthcare assistants in an orthopaedic pre-admission clinic and on a vascular ward to reduce turnaround time. POCT results were compared with the routine swabs taken for culture. The POCT assay was easy to use, the turnaround time for negative results was greatly reduced, and sensitivity was 75.0% in the pre-admission clinic and 80.0% in the vascular ward. There were 27 POCT-positive/culture-negative results, but there was no evidence of MRSA infection or colonization in these patients for at least a year post procedure. POCT for MRSA colonization performed beyond the laboratory has important advantages over laboratory-based methods and should be explored further.
对一种耐甲氧西林金黄色葡萄球菌（MRSA）聚合酶链反应检测方法（Xpert(®) MRSA，赛沛公司）进行了评估，以用于骨科入院前诊所和血管病房的医护助理进行即时检验（POCT），从而缩短周转时间。将POCT结果与用于培养的常规拭子检测结果进行比较。POCT检测方法易于使用，阴性结果的周转时间大幅缩短，在入院前诊所的灵敏度为75.0%，在血管病房为80.0%。有27例假阳性（POCT阳性/培养阴性）结果，但在这些患者术后至少一年内没有MRSA感染或定植的证据。在实验室之外进行的MRSA定植POCT相对于基于实验室的方法具有重要优势，应进一步探索。
