Coman M M, Verdenelli M C, Cecchini C, Silvi S, Orpianesi C, Boyko N, Cresci A
School of Advanced Studies, University of Camerino, Camerino, Italy; Scuola di Bioscienze e Medicina Veterinaria, University of Camerino, Camerino, Italy.
J Appl Microbiol. 2014 Aug;117(2):518-27. doi: 10.1111/jam.12544. Epub 2014 Jun 3.
Probiotic lactobacilli have a great potential to produce antimicrobial compounds that inhibit and control the microbial pathogen growth. The antimicrobial and antifungal activities of two probiotic strains, Lactobacillus rhamnosus IMC 501(®) and Lactobacillus paracasei IMC 502(®) , and their 1 : 1 combination, named SYNBIO(®) , were studied using four different methods.
Using two modified streak methods and a well diffusion method, the inhibitory activity of the probiotics and their metabolites towards six Gram-positive, nine Gram-negative pathogenic bacterial strains and eight Candida strains was tested. Antagonistic effect of probiotic Lactobacillus strains was also investigated by coculturing assay highlighting a significant inhibition of most of the pathogens tested in this study. The combination SYNBIO(®) showed a microbicidal activity against most of the strains tested in the study.
Compared with the control, most of the pathogenic bacteria and yeast were inhibited by all probiotic strains tested to various degrees.
Screening Lactobacillus strains according to their activity in various environmental conditions could precede the clinical efficacy studies for adjunct treatment with probiotics in cure of different gastrointestinal and vaginal tract infections.
益生菌乳酸杆菌具有产生抑制和控制微生物病原体生长的抗菌化合物的巨大潜力。使用四种不同方法研究了两种益生菌菌株，即鼠李糖乳杆菌IMC 501（®）和副干酪乳杆菌IMC 502（®）及其1:1组合SYNBIO（®）的抗菌和抗真菌活性。
使用两种改良划线法和一种打孔扩散法，测试了益生菌及其代谢产物对六种革兰氏阳性、九种革兰氏阴性致病细菌菌株和八种念珠菌菌株的抑制活性。还通过共培养试验研究了益生菌乳酸杆菌菌株的拮抗作用，突出显示了对本研究中测试的大多数病原体的显著抑制作用。组合SYNBIO（®）对研究中测试的大多数菌株表现出杀菌活性。
与对照相比，所有测试的益生菌菌株对大多数病原菌和酵母菌均有不同程度的抑制作用。
根据乳酸杆菌菌株在各种环境条件下的活性进行筛选，可能先于益生菌辅助治疗不同胃肠道和阴道感染的临床疗效研究。
