Faculty of Dentistry, Department of Oral Diagnostic Sciences, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Faculty of Dentistry, Research Center of Excellence for Oral Health, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Curr Microbiol. 2024 Jul 16;81(9):271. doi: 10.1007/s00284-024-03800-y.
Probiotics and prebiotics have been considered as alternative approaches for promoting health. This study aimed to investigate the anticandidal potential of various probiotic Lactobacillus strains and their cell-free supernatants (CFSs). The study assessed the impact of inulin and some fruits as prebiotics on the growth of selected probiotic strains in relation to their anticandidal activity, production of short-chain fatty acids, total phenolic content, and antioxidant activity. Results revealed variations in anticandidal activity based on the specific strains and forms of probiotics used. Non-adjusted CFSs were the most effective against Candida strains, followed by probiotic cells and adjusted CFSs (pH 7). Lacticaseibacillus rhamnosus SD4, L. rhamnosus SD11 and L. rhamnosus GG displayed the strongest anticandidal activity. Non-adjusted CFSs from L. rhamnosus SD11, L. rhamnosus SD4 and L. paracasei SD1 exhibited notable anticandidal effects. The adjusted CFSs of L. rhamnosus SD11 showed the highest anticandidal activity against all non-albicans Candida (NAC) strains, whereas the others were ineffective. Supplementation of L. rhamnosus SD11 with prebiotics, particularly 2% (w/v) mangosteen, exhibited positive results in promoting probiotic growth, short-chain fatty acids production, total phenolic contents, and antioxidant activity, and the subsequent enhancing anticandidal activity against both C. albicans and NAC strains compared to conditions without prebiotics. In conclusion, both live cells and CFSs of tested strains, particularly L. rhamnosus SD11, exhibited the best anticandidal activity. Prebiotics supplementation, especially mangosteen, enhanced probiotic growth and beneficial metabolites against Candida growth. These finding suggested that probiotics and prebiotic supplementation may be an effective alternative treatment for Candida infections.
益生菌和益生元被认为是促进健康的替代方法。本研究旨在研究各种益生菌乳杆菌菌株及其无细胞上清液（CFS）的抗真菌潜力。该研究评估了菊糖和一些水果作为益生元对选定益生菌菌株生长的影响，以及它们的抗真菌活性、短链脂肪酸的产生、总酚含量和抗氧化活性。结果表明，根据使用的特定菌株和益生菌形式，抗真菌活性存在差异。未经调整的 CFS 对念珠菌菌株最有效，其次是益生菌细胞和调整的 CFS（pH7）。鼠李糖乳杆菌 SD4、SD11 和 GG 显示出最强的抗真菌活性。未经调整的 CFS 来自 SD11、SD4 和 SD1 的鼠李糖乳杆菌表现出显著的抗真菌作用。SD11 的调整 CFS 对所有非白念珠菌（NAC）菌株表现出最高的抗真菌活性，而其他菌株则无效。用益生元，特别是 2%（w/v）山竹补充 SD11 的乳杆菌，在促进益生菌生长、短链脂肪酸产生、总酚含量和抗氧化活性方面表现出积极的结果，随后增强了对 C. albicans 和 NAC 菌株的抗真菌活性，与没有益生元的条件相比。总之，测试菌株的活细胞和 CFS ，特别是鼠李糖乳杆菌 SD11 ，表现出最好的抗真菌活性。益生元的补充，特别是山竹，增强了益生菌的生长和有益代谢物对念珠菌生长的抑制作用。这些发现表明，益生菌和益生元的补充可能是治疗念珠菌感染的有效替代方法。
