van der Klein S A S, Arora S S, Haldar S, Dhara A K, Gibbs K
Danisco Animal Nutrition & Health, IFF, Willem Einthovenstraat 4 2342 BH, Oegstgeest, The Netherlands.
Agrivet Research and Advisory Pvt Ltd., 714 Block A Lake Town, Kolkata 700089, India.
Poult Sci. 2024 Dec;103(12):104462. doi: 10.1016/j.psj.2024.104462. Epub 2024 Oct 30.
Intestinal dysbacteriosis is increasing in broilers due to the reduced use of antibiotics in feed. This study tested the effect of daily waterline administration of a dual-strain probiotic comprising Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02, on growth performance and intestinal health during a 3-step microbial challenge. In total, 900 Ross 308 males were assigned to 36 floor pens (25 birds/pen, 12 pens/treatment) in a completely randomized design. Birds were fed a corn, wheat and soybean-meal based diet. Diets were formulated in 3 phases (starter: 1 to 10; grower: 11 to 24; finisher: 25 to 42 d of age). Treatments comprised a non-challenged control (NC), challenged control (CC), and the CC supplemented with 1 × 10 colony forming units (CFU)/bird/day of the probiotic (CC+Probiotic). The challenge comprised 1 × 10 CFU/bird of Avian Pathogenic Escherichia coli on d 7, 4,000 oocysts/bird of Eimeria on d 15 and 1 × 10 CFU/bird of C. perfringens on d 18, 19 and 20. Growth performance was monitored over 42 d, blood samples, and digesta were collected and intestinal dysbacteriosis scoring was performed. Compared to NC birds, CC birds exhibited reduced BW (all phases), reduced feed intake (starter and grower phase), increased FCR (grower phase and overall; P < 0.05), reduced ileal lactic acid bacteria concentrations (d 24 and 42), and increased cecal E. coli (d 24; P < 0.05). Compared to CC birds, CC+Probiotic birds exhibited increased BW, BW gain and feed intake during grower phase (P < 0.05), increased ileal lactic acid bacteria at d 24 and 42 and reduced ileal C. perfringens at d 24, increased mucosal secretory IgA and reduced serum alpha-1-acid-glycoprotein at d 42. The overall growth performance of CC+Probiotic birds was equivalent to NC birds. These results confirm the efficacy of the dual strain probiotic for mitigating the negative effects of a multi-microbial challenge, improving gut health and growth performance in commercial broilers under dysbacteriosis challenge.
由于饲料中抗生素使用量减少，肉鸡肠道菌群失调现象日益增多。本研究测试了每日通过饮水线给予由嗜酸乳杆菌AG01和动物双歧杆菌乳酸亚种AG02组成的双菌株益生菌，对三步微生物挑战期间生长性能和肠道健康的影响。总共900只罗斯308雄性肉鸡被完全随机分配到36个地面围栏中（每围栏25只鸡，每个处理12个围栏）。鸡饲喂以玉米、小麦和豆粕为基础的日粮。日粮分三个阶段配制（开食料：1至10日龄；生长料：11至24日龄；育肥料：25至42日龄）。处理组包括未受挑战的对照组（NC）、受挑战的对照组（CC）以及添加1×10菌落形成单位（CFU）/只·天益生菌的CC组（CC + 益生菌）。挑战包括在第7天每只鸡接种1×10 CFU禽致病性大肠杆菌，在第15天每只鸡接种4000个艾美耳球虫卵囊，在第18、19和20天每只鸡接种1×10 CFU产气荚膜梭菌。在42天内监测生长性能，采集血样和消化物样本，并进行肠道菌群失调评分。与NC组鸡相比，CC组鸡体重（各阶段）降低、采食量（开食料和生长料阶段）减少、料重比升高（生长料阶段和总体；P < 0.05）、回肠乳酸菌浓度降低（第24天和42天）以及盲肠大肠杆菌增加（第24天；P < 0.05）。与CC组鸡相比，CC + 益生菌组鸡在生长料阶段体重、体重增加和采食量增加（P < 0.05），在第24天和42天回肠乳酸菌增加，在第24天回肠产气荚膜梭菌减少，在第42天黏膜分泌型IgA增加且血清α-1-酸性糖蛋白减少。CC + 益生菌组鸡的总体生长性能与NC组鸡相当。这些结果证实了双菌株益生菌在减轻多重微生物挑战的负面影响、改善肠道菌群失调挑战下商业肉鸡的肠道健康和生长性能方面的功效。
