Murphy B F, Davies D J, Morrow W, d'Apice A J
Department of Anatomical Pathology, St Vincent's Hospital, Melbourne.
Pathology. 1989 Oct;21(4):275-8. doi: 10.3109/00313028909061073.
The terminal complement complex has been implicated in the development of glomerular injury in both experimental and, indirectly, in human glomerulonephritis. Recent data suggests that the terminal complement complex in human glomerulonephritis may be in the cytolytically inactive SC5b-9 form which also contains S-protein and a recently identified protein, SP-40,40. In this study renal biopsies were examined by immunofluorescence to determine the incidence and inter-relation of deposition of the SC5b-9 components C6, C9, S-protein and SP-40,40. All components of SC5b-9 were found in arteries and arterioles, along the tubular basement membrane and in areas of glomerulosclerosis in all biopsies. This deposition was sometimes associated with C3 but never immunoglobulin deposition and correlated with the degree of renal injury. In addition, in biopsies with glomerular deposition of immunoglobulin and C3, the SC5b-9, components co-localized with the immune deposits. Glomeruli without immune deposits or glomerulosclerosis contained none of the SC5b-9 components. The incidence and pattern of distribution of SP-40,40 was similar to that of S-protein, C6 and C9 in all of cases. These data confirm that the terminal complement complex in the kidney is, at least partly, in the SC5b-9 form both in the specific immune glomerular deposition and in the "non-specific" deposition in areas of renal injury. SP-40,40 is also found in the SC5b-9 complex in all forms of renal disease.
在实验性肾小球肾炎以及间接在人类肾小球肾炎中，终末补体复合物都与肾小球损伤的发展有关。最近的数据表明，人类肾小球肾炎中的终末补体复合物可能处于细胞溶解无活性的SC5b-9形式，其还包含S蛋白和一种最近鉴定出的蛋白SP-40,40。在本研究中，通过免疫荧光检查肾活检组织，以确定SC5b-9成分C6、C9、S蛋白和SP-40,40的沉积发生率及其相互关系。在所有活检组织中，均在动脉和小动脉、沿肾小管基底膜以及肾小球硬化区域发现了SC5b-9的所有成分。这种沉积有时与C3相关，但从未与免疫球蛋白沉积相关，并且与肾损伤程度相关。此外，在有免疫球蛋白和C3肾小球沉积的活检组织中，SC5b-9成分与免疫沉积物共定位。没有免疫沉积物或肾小球硬化的肾小球不含任何SC5b-9成分。在所有病例中，SP-40,40的发生率和分布模式与S蛋白、C6和C9相似。这些数据证实，在肾脏中，终末补体复合物至少部分以SC5b-9形式存在于特异性免疫性肾小球沉积以及肾损伤区域的“非特异性”沉积中。在所有形式的肾脏疾病中，也可在SC5b-9复合物中发现SP-40,40。
