Chauhan A K, Moore T L
ProGen Biologics, Wildwood, MO 63021, USA.
Clin Exp Immunol. 2006 Sep;145(3):398-406. doi: 10.1111/j.1365-2249.2006.03135.x.
The complement regulatory (CR) proteins clusterin and vitronectin bind to the membrane attack complex (MAC) and thus prevent cytolysis. In this report, we demonstrate the presence of both of these CR proteins on MAC bound to circulating immune complexes (CIC). We measured the amount of clusterin and vitronectin on MAC in plasma, also referred to as soluble MAC (SMAC), as well as on MAC bound to CIC (MAC-CIC), using antibody directed to polymerized C9 in systemic lupus erythematosus (SLE) patients. We observed a strong correlation among the quantities of SMAC and MAC-CIC. The amount of both clusterin and vitronectin associated with MAC-CIC was two- to threefold higher in comparison to the SMAC. Patients with high levels of clusterin and vitronectin demonstrated renal involvement. We hypothesize that these complement regulatory proteins besides regulating the insertion of MAC play other critical roles, in disease pathogenesis.
补体调节（CR）蛋白聚集素和玻连蛋白可与膜攻击复合物（MAC）结合，从而防止细胞溶解。在本报告中，我们证明了这两种CR蛋白均存在于与循环免疫复合物（CIC）结合的MAC上。我们使用针对系统性红斑狼疮（SLE）患者中聚合C9的抗体，测量了血浆中MAC上聚集素和玻连蛋白的含量，血浆中的MAC也称为可溶性MAC（SMAC），以及与CIC结合的MAC（MAC-CIC）上的含量。我们观察到SMAC和MAC-CIC的量之间存在很强的相关性。与SMAC相比，与MAC-CIC相关的聚集素和玻连蛋白的量高出两到三倍。聚集素和玻连蛋白水平高的患者表现出肾脏受累。我们推测，这些补体调节蛋白除了调节MAC的插入外，在疾病发病机制中还发挥其他关键作用。
