将SP-40,40整合到补体的可溶性膜攻击复合物（SMAC，SC5b-9）中。

Incorporation of SP-40,40 into the soluble membrane attack complex (SMAC, SC5b-9) of complement.

作者信息

Choi N H, Nakano Y, Tobe T, Mazda T, Tomita M

机构信息

Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.

出版信息

Int Immunol. 1990;2(5):413-7. doi: 10.1093/intimm/2.5.413.

DOI:10.1093/intimm/2.5.413

PMID:2150757

Abstract

When SC5b-7 was prepared from the C8-depleted serum activated with inulin, it contained SP-40,40 as well as S-protein. From the densitometry of each component in SC5b-9 after SDS-PAGE, it was estimated that SC5b-9 was constituted of one molecule each of C5b, C6, C7, C8, S-protein, and SP-40,40 and two molecules of C9. SP-40,40 was depleted from normal serum with an affinity column using mouse monoclonal anti-SP-40,40 antibody. When the resulting SP-40,40-depleted serum was activated with inulin, SC5b-9 lacking SP-40,40 could be formed. S-Protein-depleted serum was also prepared with an affinity column using mouse monoclonal anti-S-protein antibody. Similarly, SC5b-9 lacking S-protein could be formed by the inulin activation of the S-protein-depleted serum. These results indicate that either SP-40,40 or S-protein should be able to form a soluble C5b-9 complex.

摘要

当用菊粉激活的C8缺失血清制备SC5b - 7时，它含有SP - 40,40以及S蛋白。通过SDS - PAGE后对SC5b - 9中各成分的光密度测定，估计SC5b - 9由一个C5b、一个C6、一个C7、一个C8、一个S蛋白、一个SP - 40,40分子以及两个C9分子组成。使用小鼠单克隆抗SP - 40,40抗体通过亲和柱从正常血清中去除SP - 40,40。当用菊粉激活所得的SP - 40,40缺失血清时，可形成缺乏SP - 40,40的SC5b - 9。同样使用小鼠单克隆抗S蛋白抗体通过亲和柱制备S蛋白缺失血清。类似地，通过S蛋白缺失血清的菊粉激活可形成缺乏S蛋白的SC5b - 9。这些结果表明，SP - 40,40或S蛋白应该都能够形成可溶性C5b - 9复合物。