a Department of Anatomy, Histology and Physiology , University of Veterinary Medicine and Pharmacy , Košice , Slovak Republic.
J Toxicol Environ Health A. 2014;77(13):747-50. doi: 10.1080/15287394.2014.890988.
This study was conducted to investigate the influence of whole-body electromagnetic radiation (EMR) on testicular parenchyma of Wistar rats. Sexually mature rats were subjected to pulsed electromagnetic field at frequency of 2.45 GHz and mean power density 2.8 mW/cm(2) by 3-h daily applications for 3 wk. Tissue samples were obtained 3 h after the last irradiation and processed by histological techniques for light and transmission electron microscopy. Testes showed apparent degenerative changes of seminiferous epithelium. The seminiferous tubules were mostly irregular in shape, and seminiferous epithelium contained a number of empty spaces of different size. Subsequently, groups of sloughed epithelial cells were often found inside the lumina of tubules. Except for relatively unchanged Sertoli cells, some locations of basal compartment of seminiferous epithelium contained shriveled Sertoli cells with dark cytoplasm. These areas showed degenerative features including necrotizing and shriveled spermatogonia surrounded by empty irregular spaces, and undulating basement membrane. The intertubular spaces were enlarged but interstitial Leydig cells did not show any marked morphological changes. Evidence demonstrates the adverse effects of EMR on testicular parenchyma in rats.
这项研究旨在探讨全身电磁场辐射（EMR）对 Wistar 大鼠睾丸实质的影响。成熟雄性大鼠接受 2.45GHz 脉冲电磁场辐射，每天 3 小时，平均功率密度为 2.8mW/cm(2)，共 3 周。末次照射后 3 小时获取组织样本，通过组织学技术进行光镜和透射电镜检查。睾丸显示出明显的曲细精管上皮变性。曲细精管形状大多不规则，上皮内含有许多不同大小的空泡。随后，经常可以在管腔内部发现脱落的上皮细胞群。除了相对不变的支持细胞外，曲细精管基底部的一些部位还含有皱缩的细胞质深染的支持细胞。这些区域表现出退行性特征，包括坏死和皱缩的精原细胞，周围有空泡不规则的空间和波状的基底膜。管间空间扩大，但间质间质细胞未见任何明显的形态变化。证据表明 EMR 对大鼠睾丸实质有不良影响。
