Almášiová Viera, Holovská Katarína, Andrašková Sandra, Cigánková Viera, Ševčíková Zuzana, Raček Adam, Andrejčáková Zuzana, Beňová Katarína, Tóth Štefan, Tvrdá Eva, Molnár Ján, Račeková Enikö
Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, the Slovak Republic.
Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, the Slovak Republic.
Histol Histopathol. 2021 Jun;36(6):685-696. doi: 10.14670/HH-18-331. Epub 2021 Mar 25.
An ever-increasing use of wireless devices over the last decades has forced scientists to clarify their impact on living systems. Since prenatal development is highly sensitive to numerous noxious agents, including radiation, we focused on the assessment of potential adverse effects of microwave radiation (MR) on testicular development. Pregnant Wistar albino rats (3 months old, weighing 282±8 g) were exposed to pulsed MR at a frequency of 2.45 GHz, mean power density of 2.8 mW/cm², and a specific absorption rate of 1.82 W/kg for 2 hours/day throughout pregnancy. Male offspring were no longer exposed to MR following birth. Samples of biological material were collected after reaching adulthood (75 days). In utero MR exposure caused degenerative changes in the testicular parenchyma of adult rats. The shape of the seminiferous tubules was irregular, germ cells were degenerated and often desquamated. The diameters of the seminiferous tubules and the height of the germinal epithelium were significantly decreased (both at ∗∗p<0.01), while the interstitial space was significantly increased (∗∗p<0.01) when compared to the controls. In the group of rats prenatally exposed to MR, the somatic and germ cells were rich in vacuoles and their organelles were often altered. Necrotizing cells were more frequent and empty spaces between Sertoli cells and germ cells were observed. The Leydig cells contained more lipid droplets. An increased Fluoro Jade - C and superoxide dismutase 2 positivity was detected in the rats exposed to MR. Our results confirmed adverse effects of MR on testicular development.
在过去几十年中，无线设备的使用日益增加，这迫使科学家们阐明其对生物系统的影响。由于产前发育对包括辐射在内的多种有害因素高度敏感，我们专注于评估微波辐射（MR）对睾丸发育的潜在不良影响。怀孕的Wistar白化大鼠（3个月大，体重282±8克）在整个孕期每天暴露于频率为2.45 GHz、平均功率密度为2.8 mW/cm²、比吸收率为1.82 W/kg的脉冲MR下2小时。雄性后代出生后不再暴露于MR。成年（75天）后收集生物材料样本。子宫内MR暴露导致成年大鼠睾丸实质发生退行性变化。生精小管形状不规则，生殖细胞退化且常发生脱落。与对照组相比，生精小管直径和生精上皮高度显著降低（均为∗∗p<0.01），而间质空间显著增加（∗∗p<0.01）。在产前暴露于MR的大鼠组中，体细胞和生殖细胞富含液泡，其细胞器常发生改变。坏死细胞更为常见，观察到支持细胞和生殖细胞之间有空隙。睾丸间质细胞含有更多脂滴。在暴露于MR的大鼠中检测到氟玉髓-C和超氧化物歧化酶2阳性增加。我们的结果证实了MR对睾丸发育的不良影响。
