Mazzulla S, Schella A, Gabriele D, Baldino N, Sesti S, Perrotta E, Costabile A, de Cindio B
Department of Biology, Ecology and Earth Science (diB.E.S.T.), University of Calabria, Arcavacata di Rende (CS), Italy.
Department of Engineering Modelling, Faculty of Chemical Engineering and Materials, University of Calabria, Arcavacata di Rende (CS), Italy.
Clin Hemorheol Microcirc. 2015;60(4):375-88. doi: 10.3233/CH-141841.
The main aim of this study was the investigation of the rheological properties of Red Blood Cells (RBCs) obtained from subjects admitted to blood donors, associated with the lipid profile of High-Density Lipoprotein Cholesterol (HDL-C), Low-Density Lipoprotein Cholesterol (LDL-C), Triglyceride (TG) and Total Cholesterol (TOT-C). The deformability index of RBCs was determined by using a Dynamic Stress Rheometer (DSR-500) under oxidative injury induced by 2.2'-azo-bis (2-amidinopropane) dihydrochloride (AAPH). An increased rigidity of RBCs in subjects with borderline level of hyperlipidemia was found, compared with the control group. In order to explain the reason for the reduced deformability, a borderline group was studied for the evaluation of anionic permeability on membrane, haemolysis and morphological changes induced by AAPH, as well as ThioBarbituric Acid Reactive Substances (TBARs) generation. Haemolysis by AAPH indicated a decrease of globular resistance in the borderline group, furthermore, the elevated values of TBARs suggested lipid peroxidative damage to the RBCs membrane associated with morphological changes and anion permeability alterations. Our study shows the correlation between biochemical and morphological parameters of RBCs and their rheological behavior during oxidative injury. Rheological studies might be useful as a clinical and diagnostic monitoring technique and provide support for further research.
本研究的主要目的是调查从献血者中招募的受试者所获得的红细胞（RBC）的流变学特性，以及与高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、甘油三酯（TG）和总胆固醇（TOT-C）的血脂谱之间的关系。红细胞的变形性指数是通过使用动态应力流变仪（DSR-500）在2,2'-偶氮二异丁脒二盐酸盐（AAPH）诱导的氧化损伤下测定的。与对照组相比，发现血脂水平处于临界值的受试者的红细胞刚性增加。为了解释变形性降低的原因，对临界值组进行了研究，以评估膜的阴离子通透性、AAPH诱导的溶血和形态变化，以及硫代巴比妥酸反应性物质（TBARs）的生成。AAPH诱导的溶血表明临界值组中球形阻力降低，此外，TBARs值升高表明红细胞膜发生脂质过氧化损伤，伴有形态变化和阴离子通透性改变。我们的研究表明红细胞的生化和形态学参数与其在氧化损伤期间的流变学行为之间存在相关性。流变学研究可能作为一种临床和诊断监测技术有用，并为进一步研究提供支持。
