Tschen J S, Tseng C N
Department of Botany, National Chung-Hsing University, Taichung, Taiwan, Republic of China.
Proc Natl Sci Counc Repub China B. 1989 Oct;13(4):258-61.
An antifungal metabolite, bacereutin, was isolated from culture filtrate of Bacillus cereus CHU 130. The bacterium was isolated from soils collected in Changhwa County, Taiwan, and was grown in soybean meal-mannitol broth for production of the antibiotic metabolite. The antibiotic metabolite was isolated by adsorption column chromatography of Amberite XAD-2 and was purified by passing through the chromatographic columns packed with Dowex 50W-X8, Sephadex LH 20 and Biogel P-2. The antibiotic metabolite was soluble in water and 87% acetone, and was slightly soluble in methanol, but was not dissolved in n-propanol, n-butanol, acetone, benzene and ethyl acetate. The antibiotic metabolite was a heat-stable and ninhydrin-positive substance. The antibiotic activities of bacereutin were tested by means of the agar-diffusion plate method. The antibiotic metabolite inhibited the growth of Saccharomyces cerevisiae CHU 1, Paecilomyces variotii CHU 6, Rhizomucor miehei CHU 40 and Fusarium oxysportum CHU 98. Bacereutin was a ninhydrin-positive antifungal antibiotic.
