Kuwayama N, Fukuda O, Koshu K, Endo S, Saito T, Takaku A
Neurol Med Chir (Tokyo). 1989 Dec;29(12):1078-84. doi: 10.2176/nmc.29.1078.
The Peltier stack, which allows quantitative measurement of cerebral blood flow (CBF) by means of thermal diffusion, has a probe too large for use in small animals. However, it is difficult to measure CBF by a thermal diffusion method involving the use of a constantan heating wire, because of heat conduction between the two gold plates. The authors developed a new thermal diffusion flow probe, using a constantan wire as a heat source rather than the Peltier stack. With the new probe, separation of the gold plates and attachment of a pair of long thermocouples minimize heat conduction between the two plates. Moreover, the probe itself is considerably smaller than that of the Peltier stack. The new probe was inserted to the subdural space of rabbits and the voltage (V; mV) was measured with an amplifier by the circuit of constant current method. CBF (F; ml/100 g/min) was measured simultaneously by the hydrogen clearance method in the adjacent cortex. A regression equation of F = 29, 111 (1/V-1/226) was obtained between 35 pairs of F and V (r = 0.92, p less than 0.001), which verified that CBF can be accurately measured with the new probe.
珀耳帖堆能通过热扩散对脑血流量（CBF）进行定量测量，但其探头对于小动物而言太大。然而，使用康铜加热丝的热扩散法测量CBF存在困难，因为两块金板之间存在热传导。作者研发了一种新的热扩散流量探头，使用康铜丝作为热源而非珀耳帖堆。使用这种新探头时，金板分离并连接一对长热电偶可使两块板之间的热传导降至最低。此外，探头本身比珀耳帖堆的探头小得多。将新探头插入兔的硬脑膜下腔，通过恒流法电路用放大器测量电压（V；mV）。在相邻皮质中用氢清除法同时测量CBF（F；ml/100 g/min）。在35对F和V之间得到回归方程F = 29,111(1/V - 1/226)（r = 0.92，p小于0.001），这证实了使用新探头能够准确测量CBF。
