Unidad de Investigación Médica-Zacatecas, Instituto Mexicano del Seguro Social, IMSS, Mexico.
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Unidad Noreste., Monterrey, Nuevo León, Mexico.
Cell Immunol. 2014 May-Jun;289(1-2):167-73. doi: 10.1016/j.cellimm.2014.03.010. Epub 2014 Apr 1.
We have hypothesized that individuals infected with Mycobacteriumtuberculosis that exhibit different patterns of immune reactivity in serial interferon (IFN)-γ release assays (IGRA's) correspond to different status within the immune spectrum of latent tuberculosis (TB). Accordingly, we analyzed the possible association between the consistent results (negative or positive) in an IGRA test and relevant immune parameters, mainly the levels of Th1 and Th17 lymphocytes and T regulatory (Treg) cells in the peripheral blood of TB case contacts. We found that individuals with a persistently positive IGRA showed increased levels of Th1 and Th17 lymphocytes upon in vitro stimulation with MTB antigens. In addition, a significant increase in the proportion of CD4+CTLA-4+ and CD4+Foxp3+ cells was detected in assays with blood samples from these individuals. Our data support that different immune phenotypes can be identified into the spectrum of latent TB, by combining different parameters of immune reactivity against MTB.
我们假设，在连续的干扰素（IFN）-γ释放试验（IGRA）中表现出不同免疫反应模式的结核分枝杆菌感染者，对应于潜伏性结核（TB）免疫谱内的不同状态。因此，我们分析了 IGRA 检测中一致（阴性或阳性）结果与相关免疫参数之间的可能关联，主要是 TB 病例接触者外周血中 Th1 和 Th17 淋巴细胞以及 T 调节（Treg）细胞的水平。我们发现，持续阳性 IGRA 的个体在体外与 MTB 抗原刺激时，Th1 和 Th17 淋巴细胞水平升高。此外，在对这些个体的血液样本进行的检测中，还检测到 CD4+CTLA-4+和 CD4+Foxp3+细胞比例的显著增加。我们的数据支持，通过结合针对 MTB 的不同免疫反应参数，可以将不同的免疫表型识别为潜伏性 TB 的谱。
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