鸡松果体细胞中cAMP的24小时振荡：cAMP在褪黑素产生的急性和昼夜节律调节中的作用。

Twenty-four hour oscillation of cAMP in chick pineal cells: role of cAMP in the acute and circadian regulation of melatonin production.

作者信息

Nikaido S S, Takahashi J S

机构信息

Interdepartmental Graduate Program in Neuroscience, Northwestern University, Evanston, Illinois 60208.

出版信息

Neuron. 1989 Nov;3(5):609-19. doi: 10.1016/0896-6273(89)90271-7.

DOI:10.1016/0896-6273(89)90271-7

PMID:2484343

Abstract

Chick pineal cells contain circadian oscillators that regulate a rhythm of melatonin biosynthesis. We explored the role of cAMP in regulating this melatonin rhythm. Chick pineal cells expressed a 24 hr oscillation of cAMP efflux with a waveform similar to that of melatonin. Elevation of cAMP in chick pineal cells stimulated melatonin. These results suggest that an oscillation of cAMP regulates the rhythm of melatonin. We investigated whether cAMP was a component of the circadian oscillator by determining the effects of 8-Br cAMP pulses on the phase of the circadian melatonin rhythm. Six hour pulses of 8-Br cAMP did not cause steady-state phase shifts of the rhythm. The acute regulation of melatonin by cAMP, the 24 hr oscillation of cAMP, and the inability of cAMP to phase-shift the melatonin rhythm strongly suggest that cAMP acts as an output signal of the circadian oscillator.

摘要

鸡松果体细胞含有调节褪黑素生物合成节律的昼夜节律振荡器。我们探究了环磷酸腺苷（cAMP）在调节这种褪黑素节律中的作用。鸡松果体细胞表现出cAMP外流的24小时振荡，其波形与褪黑素相似。鸡松果体细胞中cAMP的升高刺激了褪黑素的产生。这些结果表明，cAMP的振荡调节了褪黑素的节律。我们通过确定8-溴环磷酸腺苷（8-Br cAMP）脉冲对昼夜节律性褪黑素节律相位的影响，来研究cAMP是否是昼夜节律振荡器的一个组成部分。8-Br cAMP的6小时脉冲并未引起该节律的稳态相位偏移。cAMP对褪黑素的急性调节、cAMP的24小时振荡以及cAMP无法使褪黑素节律发生相位偏移，强烈表明cAMP作为昼夜节律振荡器的输出信号发挥作用。