Peake Matthew A, Caley Mathew, Giles Peter J, Wall Ivan, Enoch Stuart, Davies Lindsay C, Kipling David, Thomas David W, Stephens Phil
Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Tissue Engineering and Reparative Dentistry, School of Dentistry.
Wound Repair Regen. 2014 May-Jun;22(3):399-405. doi: 10.1111/wrr.12170.
There is a spectrum/continuum of adult human wound healing outcomes ranging from the enhanced (nearly scarless) healing observed in oral mucosa to scarring within skin and the nonhealing of chronic skin wounds. Central to these outcomes is the role of the fibroblast. Global gene expression profiling utilizing microarrays is starting to give insight into the role of such cells during the healing process, but no studies to date have produced a gene signature for this wound healing continuum. Microarray analysis of adult oral mucosal fibroblast (OMF), normal skin fibroblast (NF), and chronic wound fibroblast (CWF) at 0 and 6 hours post-serum stimulation was performed. Genes whose expression increases following serum exposure in the order OMF < NF < CWF are candidates for a negative/impaired healing phenotype (the dysfunctional healing group), whereas genes with the converse pattern are potentially associated with a positive/preferential healing phenotype (the enhanced healing group). Sixty-six genes in the enhanced healing group and 38 genes in the dysfunctional healing group were identified. Overrepresentation analysis revealed pathways directly and indirectly associated with wound healing and aging and additional categories associated with differentiation, development, and morphogenesis. Knowledge of this wound healing continuum gene signature may in turn assist in the therapeutic assessment/treatment of a patient's wounds.
成人伤口愈合的结果存在一个范围/连续谱，从口腔黏膜中观察到的增强（几乎无瘢痕）愈合到皮肤瘢痕形成以及慢性皮肤伤口不愈合。这些结果的核心是成纤维细胞的作用。利用微阵列进行的全基因组表达谱分析开始揭示这些细胞在愈合过程中的作用，但迄今为止尚无研究为这种伤口愈合连续谱生成基因特征。对血清刺激后0小时和6小时的成人口腔黏膜成纤维细胞（OMF）、正常皮肤成纤维细胞（NF）和慢性伤口成纤维细胞（CWF）进行了微阵列分析。血清暴露后表达按OMF＜NF＜CWF顺序增加的基因是负性/愈合受损表型（功能失调愈合组）的候选基因，而具有相反模式的基因可能与正性/优先愈合表型（增强愈合组）相关。在增强愈合组中鉴定出66个基因，在功能失调愈合组中鉴定出38个基因。过表达分析揭示了与伤口愈合和衰老直接和间接相关的途径以及与分化、发育和形态发生相关的其他类别。了解这种伤口愈合连续谱基因特征可能反过来有助于对患者伤口进行治疗评估/治疗。
