Enoch S, Wall I, Peake M, Davies L, Farrier J, Giles P, Baird D, Kipling D, Price P, Moseley R, Thomas D, Stephens P
Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Tissue Engineering and Reparative Dentistry, School of Dentistry, Cardiff University, Heath Park, Cardiff CF144XY, Wales, UK.
J Dent Res. 2009 Oct;88(10):916-21. doi: 10.1177/0022034509342979.
Oral mucosal wound-healing is characterized by rapid re-epithelialization and remodeling, with minimal scar formation. This may be attributed to the distinct phenotypic characteristics of the resident fibroblasts. To test this hypothesis, we investigated patient-matched oral mucosal and skin fibroblasts. Compared with skin fibroblasts, oral mucosal fibroblasts had longer proliferative lifespans, underwent more population doublings, and experienced senescence later, which was directly related to longer telomere lengths within oral mucosal fibroblasts. The presence of these longer telomeres was independent of telomerase expression, since both oral oral mucosal fibroblasts and skin fibroblasts were negative for active telomerase, as assessed according to the Telomeric Repeat Amplification Protocol. This study has demonstrated that, compared with skin fibroblasts, oral mucosal fibroblasts are 'younger', with a more embryonic/fetal-like phenotype that may provide a notable advantage for their ability to repair wounds in a scarless fashion.
口腔黏膜伤口愈合的特点是快速重新上皮化和重塑，瘢痕形成极少。这可能归因于驻留成纤维细胞独特的表型特征。为了验证这一假设，我们研究了患者匹配的口腔黏膜和成纤维细胞。与皮肤成纤维细胞相比，口腔黏膜成纤维细胞具有更长的增殖寿命经历更多的群体倍增，并且衰老出现得更晚，这与口腔黏膜成纤维细胞内更长的端粒长度直接相关。这些更长端粒的存在与端粒酶表达无关，因为根据端粒重复序列扩增法评估，口腔黏膜成纤维细胞和皮肤成纤维细胞的活性端粒酶均为阴性。这项研究表明，与皮肤成纤维细胞相比，口腔黏膜成纤维细胞“更年轻”，具有更类似胚胎/胎儿的表型，这可能为它们以无瘢痕方式修复伤口的能力提供显著优势。
