Sebastiani Annicchiarico L, Pagnotta R, Caravaglio N, Paparo M G
Ann Ig. 1989 Nov-Dec;1(6):1705-15.
This paper illustrates the importance of coliphages, which are specific bacteriophages for Escherichia coli strains, as viral indicators, and suggests the usefulness of a routine coliphage search in the detection of viral contamination. The samples studied consisted of 57 surface water, (46 of sea water and 11 of river water), 2 of mud taken from biological treatment plants and 3 samples of sludge. The results revealed that coliphages can be considered valid indicators of viral contamination as they show a good response to the general standards established for indicators. In particular: they are found in sludge (both untreated and treated) and in surface water (both river and sea) in higher concentrations than those of enterovirus; they show greater resistance than bacterial indicators to disinfection as, in different experimental conditions, the drop in the level does not exceed orders of magnitude they are relatively simple to identify, and are easily quantifiable by means of the MPN method, with the result that they can be used for the management of water resources. As regards the possible use of coliphages for the control of the necessary qualitative requisites of sea water for bathing, the relation with fecal coli enables us to make an assessment of coliphages corresponding to 100 fecal coli/100 ml. This levels is equivalent to 0.95 so that a suitable guide line for water for bathing purposes could for example be established at 10 coliphages/1. Although coliphages may be considered good indicators of viral contamination this clearly does not mean that they can replace classic bacterial indicators but rather, that they can provide useful additional information, particularly in those cases where a rapid drop in bacterial content may conceal high risk health and hygiene conditions.
本文阐述了作为病毒指示物的大肠杆菌噬菌体(即针对大肠杆菌菌株的特异性噬菌体)的重要性,并表明在检测病毒污染中常规检测大肠杆菌噬菌体的实用性。所研究的样本包括57份地表水(46份海水和11份河水)、2份取自生物处理厂的泥浆样本以及3份污泥样本。结果显示,大肠杆菌噬菌体可被视为病毒污染的有效指示物,因为它们对既定的指示物通用标准表现出良好的响应。具体而言:在污泥(未经处理的和经处理的)以及地表水(河水和海水)中,它们的浓度高于肠道病毒;与细菌指示物相比,它们对消毒具有更强的抗性,因为在不同实验条件下,其数量的下降不超过几个数量级;它们相对易于识别,并且通过最大可能数法易于定量,因此可用于水资源管理。关于大肠杆菌噬菌体在控制海水浴场所需水质要求方面的可能用途,与粪大肠菌的关系使我们能够评估相当于100个粪大肠菌/100毫升的大肠杆菌噬菌体数量。这个水平相当于0.95,因此例如可以将用于沐浴目的的水的合适指导标准设定为10个大肠杆菌噬菌体/升。尽管大肠杆菌噬菌体可被视为病毒污染的良好指示物,但这显然并不意味着它们可以取代传统的细菌指示物,而是意味着它们可以提供有用的额外信息,特别是在细菌含量快速下降可能掩盖高健康和卫生风险状况的情况下。