Favier A, Ruffieux D
Laboratoire de Biochimie C, Centre Hospitalier Régional de Grenoble, France.
Biol Trace Elem Res. 1988 Dec;18:145-60. doi: 10.1007/BF02917499.
The authors present a simple and rapid method for assaying ultrafilterable copper (Cu UF) and albumin-bound copper (Cu ALB). It is based on the ultrafiltration of serum in the presence of ethylenediaminetetraacetic acid (EDTA), used to prevent adsorption on the membranes. EDTA at 0.4 g/L has no effect on the equilibrium of serum copper vectors and enables Cu UF to be assayed by flameless atomic absorption. EDTA at 2 g/L is used to assay total exchangeable copper (CU EXC) (ultrafilterable + albumin-bound). The evaluation criteria of the method are furnished, as are the normal values in healthy subjects: 14.6 micrograms/L for Cu UF and 87 micrograms/L for Cu EXC. Finally, the usefulness of the methods described here for the diagnosis of Wilson's and Menkes' disease was demonstrated.
作者提出了一种简单快速的方法来测定可超滤铜（Cu UF）和白蛋白结合铜（Cu ALB）。该方法基于在乙二胺四乙酸（EDTA）存在下对血清进行超滤，EDTA用于防止铜吸附在膜上。0.4 g/L的EDTA对血清铜载体的平衡没有影响，可通过无火焰原子吸收法测定Cu UF。2 g/L的EDTA用于测定总可交换铜（CU EXC）（可超滤铜+白蛋白结合铜）。文中给出了该方法的评估标准以及健康受试者的正常值：Cu UF为14.6微克/升，Cu EXC为87微克/升。最后，证明了本文所述方法对威尔逊病和门克斯病诊断的实用性。
