Song Hui, Nan Zhibiao
College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China.
Yi Chuan. 2014 Feb;36(2):152-68. doi: 10.3724/sp.j.1005.2014.00152.
WRKY gene family plays important roles in plant by involving in transcriptional regulations during various physiologically processes such as development, metabolism and responses to biotic and abiotic stresses. WRKY genes have been identified in various plants. However, only few WRKY genes in Medicago truncatula have been identified with systematic analysis and comparison. In this study, we identified 93 WRKY genes through analyses of M. truncatula genome. These genes include 19 type-I genes, 49 type II genes and 13 type-III genes, and 12 non-regular type genes. All of these genes were characterized through analyses of gene duplication, chromosomal locations, structural diversity, conserved protein motifs and phylogenetic relations. The results showed that 11 times of gene duplication event occurred in WRKY gene family involving 24 genes. WRKY genes, containing 6 gene clusters, are unevenly distributed into chromosome 1 to 6, and there is the purifying selection pressure in WRKY group III genes.
WRKY基因家族通过参与植物发育、代谢以及对生物和非生物胁迫的响应等各种生理过程中的转录调控，在植物中发挥着重要作用。WRKY基因已在多种植物中被鉴定出来。然而，只有少数蒺藜苜蓿WRKY基因经过了系统的分析和比较。在本研究中，我们通过对蒺藜苜蓿基因组的分析鉴定出了93个WRKY基因。这些基因包括19个I型基因、49个II型基因和13个III型基因，以及12个非规则型基因。所有这些基因都通过基因复制、染色体定位、结构多样性、保守蛋白基序和系统发育关系分析进行了表征。结果表明，WRKY基因家族发生了11次基因复制事件，涉及24个基因。WRKY基因包含6个基因簇，不均匀地分布在第1至6号染色体上，并且WRKY III组基因存在纯化选择压力。
