Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China.
Int J Mol Sci. 2023 May 30;24(11):9519. doi: 10.3390/ijms24119519.
The WRKY transcription factor family plays a vital role in plant development and environmental response. However, the information of genes at the genome-wide level is rarely reported in . In this study, we identified and renamed 86 genes, which were further classified into three groups through phylogenetic analysis. Most of these genes were clustered and distributed on eight chromosomes. Multiple sequence alignment revealed that the conserved domain (WRKYGQK) of the CkWRKYs was basically consistent, but there were also six variation types (WRKYGKK, GRKYGQK, WRMYGQK, WRKYGHK, WKKYEEK and RRKYGQK) that appeared. The motif composition of the CkWRKYs was quite conservative in each group. In general, the number of genes gradually increased from lower to higher plant species in the evolutionary analysis of 28 species, with some exceptions. Transcriptomics data and RT-qPCR analysis showed that the in different groups were involved in abiotic stresses and ABA response. Our results provided a basis for the functional characterization of the CkWRKYs involved in stress resistance in .
WRKY 转录因子家族在植物发育和环境响应中起着至关重要的作用。然而，在 中很少有关于基因的全基因组水平信息的报道。在这项研究中，我们鉴定并重新命名了 86 个基因，通过系统发育分析进一步将它们分为三组。这些基因大多数聚集并分布在 8 条染色体上。多重序列比对表明，CkWRKYs 的保守结构域（WRKYGQK）基本一致，但也存在六种变异类型（WRKYGKK、GRKYGQK、WRMYGQK、WRKYGHK、WKKYEEK 和 RRKYGQK）。在每个组中，CkWRKYs 的基序组成都相当保守。一般来说，在 28 个物种的进化分析中，从低等植物到高等植物的基因数量逐渐增加，但也存在一些例外。转录组学数据和 RT-qPCR 分析表明，不同组中的基因参与了非生物胁迫和 ABA 反应。我们的研究结果为参与 胁迫抗性的 CkWRKYs 的功能表征提供了基础。
