小麦RNA聚合酶II与非组蛋白染色体蛋白（NHCP）复合物的转录活性。

Transcriptional activity of a complex of wheat RNA polymerase II and nonhistone chromosomal protein (NHCP).

作者信息

Dullin P, Galbas M, Chodynicka-Kordus G, Fabisz-Kijowska A, Walerych W

机构信息

Institute of Biochemistry, Agriculture University, Poznań, Poland.

出版信息

Acta Biochim Pol. 1989;36(3-4):263-74.

PMID:2484736

Abstract

A protein stimulating RNA polymerase activity that has been isolated and partially characterized previously (Walerych, W., Fabisz-Kijowska, A. et al., 1982, Phytochemistry, 21, 1495-1507) was subjected to investigations on its possible involvement in transcription process. Affinity chromatography revealed that this protein strongly binds to RNA polymerase-Sepharose column. The NHCP protects the enzyme against thermal inactivation, while the polymerase stabilizes the NHCP and maintains its ability to stimulate DNA transcription. The NHCP increases the affinity of RNA polymerase to DNA. Upon its addition, longer transcripts are obtained when a zein gene is used as a template for in vitro transcription. The RNA polymerase-NHCP complex formed has greater affinity to DNA than free enzyme. This seems to be due to enhanced sensitivity of bound enzyme to signals encoded in DNA sequences.

摘要

一种先前已被分离并部分表征的刺激RNA聚合酶活性的蛋白质（Walerych, W., Fabisz-Kijowska, A.等人，1982年，《植物化学》，21卷，1495 - 1507页），针对其在转录过程中可能的参与情况进行了研究。亲和层析显示该蛋白质与RNA聚合酶 - 琼脂糖柱紧密结合。非组蛋白染色体蛋白（NHCP）保护该酶免受热失活，而聚合酶则稳定NHCP并维持其刺激DNA转录的能力。NHCP增加了RNA聚合酶对DNA的亲和力。加入NHCP后，以玉米醇溶蛋白基因为模板进行体外转录时可获得更长的转录本。所形成的RNA聚合酶 - NHCP复合物对DNA的亲和力比游离酶更高。这似乎是由于结合的酶对DNA序列中编码的信号敏感性增强所致。