Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2014 Mar;17(3):196-200.
OBJECTIVE(S): Skin extracellular matrix, which contains type I and type III collagens, is involved in skin development. The aim of this study was to investigate type III collagen distribution pattern as well as its changes during pre and post-natal skin morphogenesis in rats.
Ventral skins of Wistar rat embryos at different stages from 10 to 20 gestational day (E10-E20) and also one month and one year post natal rat pups were fixed in normalin, embedded in paraffin and 5 µm thick sections were incubated with Anti type III collagen antibody. In order to detect staining intensity, the reactions were observed and graded by three examiners separately. Kruskal-Wallis non-parametric statistical test and SPSS software version 11.5 were used to compare differences between samples.
Immunoreactivity of type III collagen was distributed weakly in the mesenchymal connective tissue on day 10 (E10). The observed reaction was increased onE12 and E14. This reaction was clear in basement membrane, relatively intensive in dermal papillae and moderate in dermal reticularis on E14. This immunoreactivity pattern was increased afterward on E16, not changed on E18 and decreased in dermal reticularis on E20. The density of collagen type III in dermal papillae and dermal reticularis in skin of one year old rats were decreased comparing to one month old rats.
Our results showed that type III collagen is expressed and timely regulated during pre and post natal rat skin morphogenesis.
皮肤细胞外基质含有 I 型和 III 型胶原，参与皮肤发育。本研究旨在研究 III 型胶原的分布模式及其在大鼠产前和产后皮肤形态发生过程中的变化。
将来自 10 至 20 天妊娠（E10-E20）的不同阶段的 Wistar 大鼠胚胎的腹侧皮肤以及出生后 1 个月和 1 年的大鼠幼崽的皮肤用福尔马林固定，嵌入石蜡中，并将 5µm 厚的切片用抗 III 型胶原抗体孵育。为了检测染色强度，由三名检查者分别观察和分级反应。使用 Kruskal-Wallis 非参数统计检验和 SPSS 软件版本 11.5 来比较样品之间的差异。
III 型胶原的免疫反应性在第 10 天（E10）的间充质结缔组织中分布较弱。在 E12 和 E14 观察到的反应增加。在 E14 时，这种反应在基底膜中清晰，在真皮乳头中相对强烈，在真皮网状层中中度强烈。此后，这种免疫反应性增加，在 E16 不变，在 E18 减少，在 E20 减少真皮网状层。与 1 个月大的大鼠相比，1 岁大鼠皮肤的真皮乳头和真皮网状层的 III 型胶原密度降低。
我们的结果表明，III 型胶原在大鼠产前和产后皮肤形态发生过程中表达并进行适时调节。
