在酶联免疫吸附测定（ELISA）试验中作为包被抗原的多形拟杆菌细胞表面成分
The cell-surface components of Bacteroides thetaiotaomicron as coating antigens in ELISA test.
作者信息
Rokosz A, Grzybowski J, Kaca W, Meisel-Mikołajczyk F
机构信息
Department of Bacteriology and Immunology, Institute of Biostructure, Medical Academy, Warsaw, Poland.
出版信息
Acta Microbiol Pol. 1989;38(3-4):285-91.
Cell-surface antigens were extracted out of three Bacteroides thetaiotaomicron strains of different origin. Lipopolysaccharides, their fractions, L1 preparations and capsular antigen were obtained. All substances were tested as coating antigens in ELISA test against antibacterial rabbit immune sera. The highest absorbances were observed with lipopolysaccharides (LPS), their polysaccharide fractions (PS) and capsular material (CPS). Lipopolysaccharides after nuclease treatment (N-LPS), free from nucleic acids, were more active than crude phenol-water extracts (PW-LPS).
从三株不同来源的多形拟杆菌中提取细胞表面抗原。获得了脂多糖、其组分、L1制剂和荚膜抗原。所有物质都作为包被抗原用于ELISA试验，以检测抗兔抗菌免疫血清。观察到脂多糖（LPS）、其多糖组分（PS）和荚膜物质（CPS）的吸光度最高。经核酸酶处理后不含核酸的脂多糖（N-LPS）比粗酚水提取物（PW-LPS）更具活性。
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