Meisel-Mikolajczyk F, Rokosz A, Kaca W
Department of Bacteriology and Immunology, Medical Academy, Warsaw, Poland.
Eur J Epidemiol. 1989 Dec;5(4):486-96. doi: 10.1007/BF00140146.
Three strains of B. thetaiotaomicron of different origin were investigated. Lipopolysaccharides were extracted from the studied strains using a phenol-water method. The best purification of LPS was achieved by digestion with nuclease and subsequent ultracentrifugation. Capsular material (CPS) was obtained from the most heavily encapsulated strain. The preparation were analyzed chemically, and their serological activity was determined. All antigens were active with homologous antibacterial sera in immunodiffusion, crossed immunoelectrophoresis, and passive hemagglutination tests. In the CPS equal amounts of saccharides and proteins were detected. All antigens were analyzed by polyacrylamide gel electrophoresis with SDS. Capsular antigen slowly migrated in the gel in the form of a single band. Migration pattern of lipopolysaccharides of the studied B. thetaiotaomicron strain was characteristic for S-type LPS.
对三株不同来源的嗜内脏拟杆菌进行了研究。使用酚水法从所研究的菌株中提取脂多糖。通过核酸酶消化和随后的超速离心实现了脂多糖的最佳纯化。从包囊最厚的菌株中获得荚膜物质（CPS）。对制剂进行化学分析，并测定其血清学活性。在免疫扩散、交叉免疫电泳和被动血凝试验中，所有抗原与同源抗菌血清均呈阳性反应。在CPS中检测到等量的糖类和蛋白质。所有抗原均通过SDS聚丙烯酰胺凝胶电泳进行分析。荚膜抗原在凝胶中以单一条带的形式缓慢迁移。所研究的嗜内脏拟杆菌菌株的脂多糖迁移模式为S型脂多糖的特征。
