Nagamori S, Fujise K, Hasumura S, Homma S, Sujino H, Matsuura T, Shimizu K, Niiya M, Kameda H
First Department of Internal Medicine, Jikei University School of Medicine.
Hum Cell. 1988 Dec;1(4):382-90.
Liver cells have many functions, and one of which is a production of plasma proteins. Therefore, studies on synthesis and production of plasma proteins from hepatocytes are very important for the recognition of various hepatic dysfunctions, clinically. Of late years, a lot of the complex mechanism of protein synthesis and--secretion was elucidated by using a technique of liver cell culture, for example, primary monolayer culture by freshly isolated hepatocytes and cloned cell culture derived from hepatocellular carcinoma. This paper described the results of our observations and other researchers, and then discussed the point of production of human major plasma proteins using the above culture methods, such as albumin, alpha-fetoprotein and transferrin. Furthermore, we showed statistically that half of twenty-six human hepatoma cell lines established until 1988 in Japan, had already lost their secretory potencies of major plasma proteins in vitro.
肝细胞有多种功能，其中之一是产生血浆蛋白。因此，研究肝细胞合成和产生血浆蛋白对于临床上识别各种肝功能障碍非常重要。近年来，通过使用肝细胞培养技术，例如用新鲜分离的肝细胞进行原代单层培养以及源自肝细胞癌的克隆细胞培养，阐明了许多蛋白质合成和分泌的复杂机制。本文描述了我们的观察结果以及其他研究人员的结果，然后讨论了使用上述培养方法产生人类主要血浆蛋白的要点，如白蛋白、甲胎蛋白和转铁蛋白。此外，我们通过统计表明，截至1988年在日本建立的26个人肝癌细胞系中，有一半在体外已经失去了主要血浆蛋白的分泌能力。
