Tonon Federica, Farra Rossella, Zennaro Cristina, Pozzato Gabriele, Truong Nhung, Parisi Salvatore, Rizzolio Flavio, Grassi Mario, Scaggiante Bruna, Zanconati Fabrizio, Bonazza Deborah, Grassi Gabriele, Dapas Barbara
Department of Medical, Surgical and Health Sciences, University of Trieste, Cattinara Hospital, Strada di Fiume, 447, I 34149 Trieste, Italy.
Stem Cell Research and Application Laboratory, VNUHCM, University of Science, Ho Chi Minh City 72711, Vietnam.
Pharmaceuticals (Basel). 2021 Aug 16;14(8):803. doi: 10.3390/ph14080803.
Hepatocellular carcinoma (HCC) is the sixth most common type of tumor and the second leading cause of tumor-related death worldwide. Liver cirrhosis is the most important predisposing factor for HCC. Available therapeutic approaches are not very effective, especially for advanced HCC, which is the most common form of the disease at diagnosis. New therapeutic strategies are therefore urgently needed. The use of animal models represents a relevant tool for preclinical screening of new molecules/strategies against HCC. However, several issues, including animal husbandry, limit the use of current models (rodent/pig). One animal model that has attracted the attention of the scientific community in the last 15 years is the zebrafish. This freshwater fish has several attractive features, such as short reproductive time, limited space and cost requirements for husbandry, body transparency and the fact that embryos do not show immune response to transplanted cells. To date, two different types of zebrafish models for HCC have been developed: the transgenic zebrafish and the zebrafish xenograft models. Since transgenic zebrafish models for HCC have been described elsewhere, in this review, we focus on the description of zebrafish xenograft models that have been used in the last five years to test new molecules/strategies against HCC.
肝细胞癌（HCC）是全球第六大常见肿瘤类型，也是肿瘤相关死亡的第二大主要原因。肝硬化是HCC最重要的诱发因素。现有的治疗方法效果不佳，尤其是对于晚期HCC，而晚期HCC是该疾病在诊断时最常见的形式。因此，迫切需要新的治疗策略。动物模型的使用是针对HCC进行新分子/策略临床前筛选的一种相关工具。然而，包括动物饲养在内的几个问题限制了当前模型（啮齿动物/猪）的使用。在过去15年中引起科学界关注的一种动物模型是斑马鱼。这种淡水鱼具有几个吸引人的特点，如繁殖时间短、饲养所需空间和成本有限、身体透明以及胚胎对移植细胞不表现出免疫反应。迄今为止，已经开发出两种不同类型的用于HCC的斑马鱼模型：转基因斑马鱼模型和斑马鱼异种移植模型。由于转基因斑马鱼HCC模型已在其他地方进行了描述，在本综述中，我们重点描述过去五年中用于测试针对HCC的新分子/策略的斑马鱼异种移植模型。
