Szpirer J, Szpirer C
Cell. 1975 Sep;6(1):53-60. doi: 10.1016/0092-8674(75)90073-2.
Hybrids between mouse hepatoma cells (which secrete several serum proteins) and mouse or rat fibroblasts (which do not secrete these proteins) produce transferrin and the third component of complement (C3) like the parental hepatoma cells, while they do not secrete either albumin or alpha-fetoprotein (AFP). This lack of albumin and AFP secretion is probably due to a lack of synthesis, rather than to a simple defect in secretion. The cessation of albumin and AFP production is not dependent upon the parental fibroblast nor upon the selection conditions; it is best explained by a shut-off synthesis and could thus reflect the existence of a regulatory mechanism. This would imply a difference between the control of albumin and AFP synthesis and that of transferrin and C3 synthesis. On the other hand, in agreement with Peterson and Weiss (1972), hybrids between rat hepatoma cells and mouse fibroblasts continue to product rat albumin. This suggests that the mouse hepatoma cells differ from the rat hepatoma cells in the way they control albumin production.
小鼠肝癌细胞（分泌多种血清蛋白）与小鼠或大鼠成纤维细胞（不分泌这些蛋白）之间的杂交细胞，像亲代肝癌细胞一样产生转铁蛋白和补体第三成分（C3），而它们既不分泌白蛋白也不分泌甲胎蛋白（AFP）。白蛋白和AFP分泌的缺乏可能是由于合成的缺乏，而不是简单的分泌缺陷。白蛋白和AFP产生的停止不依赖于亲代成纤维细胞，也不依赖于选择条件；最好用合成关闭来解释，因此可能反映了一种调节机制的存在。这意味着白蛋白和AFP合成的控制与转铁蛋白和C3合成的控制之间存在差异。另一方面，与彼得森和韦斯（1972年）的研究一致，大鼠肝癌细胞与小鼠成纤维细胞之间的杂交细胞继续产生大鼠白蛋白。这表明小鼠肝癌细胞在控制白蛋白产生的方式上与大鼠肝癌细胞不同。
