Yu Hai, Lau Kam, Thon Vireak, Autran Chloe A, Jantscher-Krenn Evelyn, Xue Mengyang, Li Yanhong, Sugiarto Go, Qu Jingyao, Mu Shengmao, Ding Li, Bode Lars, Chen Xi
Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, CA 95616 (USA).
Angew Chem Int Ed Engl. 2014 Jun 23;53(26):6687-91. doi: 10.1002/anie.201403588. Epub 2014 May 21.
Two novel synthetic α2-6-linked disialyl hexasaccharides, disialyllacto-N-neotetraose (DSLNnT) and α2-6-linked disialyllacto-N-tetraose (DS'LNT), were readily obtained by highly efficient one-pot multienzyme (OPME) reactions. The sequential OPME systems described herein allowed the use of an inexpensive disaccharide and simple monosaccharides to synthesize the desired complex oligosaccharides with high efficiency and selectivity. DSLNnT and DS'LNT were shown to protect neonatal rats from necrotizing enterocolitis (NEC) and are good therapeutic candidates for preclinical experiments and clinical application in treating NEC in preterm infants.
通过高效一锅多酶（OPME）反应很容易获得两种新型合成α2-6连接的二唾液酸己糖，二唾液酸乳糖-N-新四糖（DSLNnT）和α2-6连接的二唾液酸乳糖-N-四糖（DS'LNT）。本文所述的连续OPME系统允许使用廉价的二糖和简单的单糖高效且选择性地合成所需的复杂寡糖。DSLNnT和DS'LNT已被证明可保护新生大鼠免受坏死性小肠结肠炎（NEC）的侵害，是早产儿NEC临床前实验和临床治疗的良好候选药物。
