Kim S Y, Stoetter H, Heimpel H
Korean J Intern Med. 1987 Jul;2(2):201-8. doi: 10.3904/kjim.1987.2.2.201.
The effects of interferons (IFN) on in vitro differentiation of B-lymphocytes were studied. Peripheral lymphocytes from normal subjects were cultivated under polyclonal activator pokeweed mitogen (PWN) or Epstein-Barr virus (EBV) stimulation. The secreted Ig in the culture supernatants were measured for IgM by ELISA method. To determine the cellular level of IFN action T-cell enriched fraction (Te) or B-cell enriched fraction (Be) were preincubated with IFN prior to recombination culture. IFN had modulatory activities on Ig production; at low to moderately high doses (10–1000 U/ml of IFN-alpha or 12–120 U/ml of IFN-gamma) stimulating when IFN was added until 48 hr after the start of the culture, while after 72 hr from culture start IFN suppressed Ig production. Preincubation of Be-cells with moderately high doses of IFN (120 U/ml of IFN-gamma or 1000 U/ml of IFN-alpha) prior to PWM-stimulation suppressed Ig production. Likewise, in EBV-stimulated culture, high dose IFN suppressed Ig production. But low dose of IFN enhanced ig production in EBV-stimulated culture. Preincubation of Te-cells with IFN prior to PWM-stimulation with Be-cells enhanced the Ig production. The T-cell subset analysis at the end of these culture showed enhanced ratio of T-helper cell relative to T-suppressor cells, suggesting increased T-helper cell proliferation after incubation with IFN. Thus, it is concluded that IFNs have modulatory activities on B-cell differentiation. The mechanism seems to be direct effects on B-cells (in PWM and EBV system) as well as through T-helper cell mediation (PWM system). The IFN-gamma showed more potent (2-to 6-fold) stimulatory activities than IFN-alpha.
研究了干扰素（IFN）对B淋巴细胞体外分化的影响。将正常受试者的外周淋巴细胞在多克隆激活剂商陆有丝分裂原（PWM）或爱泼斯坦-巴尔病毒（EBV）刺激下培养。通过ELISA法测定培养上清液中分泌的IgM。为了确定IFN作用的细胞水平，在重组培养前，将富含T细胞的组分（Te）或富含B细胞的组分（Be）与IFN预孵育。IFN对Ig产生具有调节活性；在低至中等高剂量（10–1000 U/ml的IFN-α或12–120 U/ml的IFN-γ）时，在培养开始后添加IFN直至48小时具有刺激作用，而在培养开始72小时后IFN抑制Ig产生。在PWM刺激前，将Be细胞与中等高剂量的IFN（120 U/ml的IFN-γ或1000 U/ml的IFN-α）预孵育可抑制Ig产生。同样，在EBV刺激的培养中，高剂量IFN抑制Ig产生。但低剂量IFN可增强EBV刺激培养中的Ig产生。在与Be细胞进行PWM刺激前，将Te细胞与IFN预孵育可增强Ig产生。这些培养结束时的T细胞亚群分析显示，辅助性T细胞与抑制性T细胞的比例增加，提示与IFN孵育后辅助性T细胞增殖增加。因此，得出结论，IFN对B细胞分化具有调节活性。其机制似乎是对B细胞的直接作用（在PWM和EBV系统中）以及通过辅助性T细胞介导（PWM系统）。IFN-γ显示出比IFN-α更强（2至6倍）的刺激活性。
