爱泼斯坦-巴尔病毒激活人B淋巴细胞的特征。
Characteristics of Epstein-Barr virus activation of human B lymphocytes.
作者信息
Bird A G, Britton S, Ernberg I, Nilsson K
出版信息
J Exp Med. 1981 Sep 1;154(3):832-9. doi: 10.1084/jem.154.3.832.
Abstract
Epstein-Barr virus (EBV) will infect at least every third cell if exposed in vitro to an extensively purified B cell population from human peripheral blood. About 10% of such infected cells will be driven into immunoglobulin synthesis and secretion, as judged by the indirect protein A plaque assay. The appearance of EB nuclear antigen, de novo DNA synthesis, and immunoglobulin secretion are linked phenomena accompanying infection as judged by viral dilution experiments, which yield kinetics of a one-hit order. Induction of immunoglobulin secretion in B cells by EBV requires de novo synthesis of DNA, and consequently, nontransforming EBV (P3HR1) will not induce immunoglobulin secretion and will also specifically block such induction from subsequently added EBV. The termination of immunoglobulin induction by EBV in short-term cultures appears to be T cell dependent.
摘要
如果在体外将爱泼斯坦-巴尔病毒（EBV）暴露于来自人外周血的高度纯化的B细胞群体，它将至少感染每三个细胞中的一个。通过间接蛋白A空斑试验判断，约10%的此类感染细胞将被驱动进行免疫球蛋白的合成和分泌。通过病毒稀释实验判断，EB核抗原的出现、DNA的从头合成和免疫球蛋白的分泌是伴随感染的相关现象，这些实验产生了一级动力学。EBV诱导B细胞分泌免疫球蛋白需要DNA的从头合成，因此，非转化性EBV（P3HR1）不会诱导免疫球蛋白分泌，并且还会特异性阻断随后添加的EBV的这种诱导作用。在短期培养中，EBV诱导免疫球蛋白的终止似乎依赖于T细胞。
相似文献
1
Characteristics of Epstein-Barr virus activation of human B lymphocytes.爱泼斯坦-巴尔病毒激活人B淋巴细胞的特征。
J Exp Med. 1981 Sep 1;154(3):832-9. doi: 10.1084/jem.154.3.832.
2
Lymphoblastoid transformation and kinetics of appearance of viral nuclear antigen (EBNA) in cord-blood lymphocytes infected by Epstein-Barr Virus (EBV).爱泼斯坦-巴尔病毒（EBV）感染的脐血淋巴细胞中的淋巴母细胞转化及病毒核抗原（EBNA）出现的动力学
Int J Cancer. 1975 Mar 15;15(3):377-84. doi: 10.1002/ijc.2910150303.
3
Infection of mouse lymphocytes by Epstein-Barr virus. II. Stimulation of cellular DNA synthesis by EBV in the absence of EBNA induction and cell transformation.爱泼斯坦-巴尔病毒对小鼠淋巴细胞的感染。II. 在无EBNA诱导和细胞转化情况下，EBV对细胞DNA合成的刺激作用
Virology. 1984 Feb;133(1):211-5. doi: 10.1016/0042-6822(84)90441-0.
4
Limiting dilution analysis of Epstein-Barr virus-induced immunoglobulin production by human B cells.爱泼斯坦-巴尔病毒诱导人B细胞产生免疫球蛋白的有限稀释分析。
J Exp Med. 1983 Jan 1;157(1):1-14. doi: 10.1084/jem.157.1.1.
5
The effect of tinidazole on primary EBV infection and immunocompetence.替硝唑对原发性EB病毒感染及免疫能力的影响。
Scand J Infect Dis. 1984;16(1):17-23. doi: 10.3109/00365548409068404.
6
Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma.在爱泼斯坦-巴尔病毒阳性弥漫性大B细胞淋巴瘤患者中，病毒载量高时T细胞对爱泼斯坦-巴尔病毒的反应增强。
Leuk Lymphoma. 2015 Apr;56(4):1072-8. doi: 10.3109/10428194.2014.938326. Epub 2014 Aug 13.
7
Epstein-Barr virus (EBV) antigens processed and presented by B cells, B blasts, and macrophages trigger T-cell-mediated inhibition of EBV-induced B-cell transformation.由B细胞、B母细胞和巨噬细胞加工并呈递的爱泼斯坦-巴尔病毒（EBV）抗原会触发T细胞介导的对EBV诱导的B细胞转化的抑制作用。
J Virol. 1990 Mar;64(3):1398-401. doi: 10.1128/JVI.64.3.1398-1401.1990.
8
Epstein-Barr virus (EBV) receptors, complement receptors, and EBV infectibility of different lymphocyte fractions of human peripheral blood. II. Epstein-Barr virus studies.爱泼斯坦-巴尔病毒（EBV）受体、补体受体以及人外周血不同淋巴细胞亚群的EBV感染性。II. 爱泼斯坦-巴尔病毒研究
Cell Immunol. 1978 Jan;35(1):43-58. doi: 10.1016/0008-8749(78)90125-9.
9
10
The effects of cyclosporin A on the early responses of B and T cells to Epstein-Barr virus infection.环孢菌素A对B细胞和T细胞对爱泼斯坦-巴尔病毒感染早期反应的影响。
Immunol Lett. 1986 Oct;13(4):173-8. doi: 10.1016/0165-2478(86)90051-9.
引用本文的文献
1
Assessment of the effect of TLR7/8, TLR9 agonists and CD40 ligand on the transformation efficiency of Epstein-Barr virus in human B lymphocytes by limiting dilution assay.通过有限稀释分析评估 TLR7/8、TLR9 激动剂和 CD40 配体对人 B 淋巴细胞中 EBV 转化效率的影响。
Cytotechnology. 2014 Jan;66(1):95-105. doi: 10.1007/s10616-013-9542-x. Epub 2013 Feb 13.
2
Establishment of Epstein-Barr virus growth-transformed lymphoblastoid cell lines.爱泼斯坦-巴尔病毒生长转化的淋巴母细胞系的建立。
J Vis Exp. 2011 Nov 8(57):3321. doi: 10.3791/3321.
3
Different individual immune responses elicited by in vitro immunization.不同个体经体外免疫诱导产生的免疫应答。
Cytotechnology. 2002 Nov;40(1-3):161-5. doi: 10.1023/A:1023992506427.
4
An efficient method for the rapid establishment of Epstein-Barr virus immortalization of human B lymphocytes.一种快速建立人B淋巴细胞爱泼斯坦-巴尔病毒永生化的有效方法。
Cell Prolif. 2003 Aug;36(4):191-7. doi: 10.1046/j.1365-2184.2003.00276.x.
5
Epstein-Barr virus nuclear proteins EBNA-3A and EBNA-3C are essential for B-lymphocyte growth transformation.爱泼斯坦-巴尔病毒核蛋白EBNA-3A和EBNA-3C对B淋巴细胞生长转化至关重要。
J Virol. 1993 Apr;67(4):2014-25. doi: 10.1128/JVI.67.4.2014-2025.1993.
6
Epstein-Barr virus recombinants from overlapping cosmid fragments.来自重叠黏粒片段的爱泼斯坦-巴尔病毒重组体。
J Virol. 1993 Dec;67(12):7298-306. doi: 10.1128/JVI.67.12.7298-7306.1993.
7
An Epstein-Barr virus with a 58-kilobase-pair deletion that includes BARF0 transforms B lymphocytes in vitro.一种带有58千碱基对缺失（包括BARF0）的爱泼斯坦-巴尔病毒可在体外转化B淋巴细胞。
J Virol. 1994 Mar;68(3):1449-58. doi: 10.1128/JVI.68.3.1449-1458.1994.
8
An efficient method for routine Epstein-Barr virus immortalization of human B lymphocytes.一种用于人B淋巴细胞常规爱泼斯坦-巴尔病毒永生化的有效方法。
In Vitro Cell Dev Biol Anim. 1995 Feb;31(2):156-9. doi: 10.1007/BF02633976.
9
X-linked lymphoproliferative syndrome. Natural history of the immunodeficiency.X连锁淋巴增殖综合征。免疫缺陷的自然史。
J Clin Invest. 1983 Jun;71(6):1765-78. doi: 10.1172/jci110932.
10
Frequencies of the separate human B cell subsets activatable to Ig secretion by Epstein-Barr virus and pokeweed mitogen.可被爱泼斯坦-巴尔病毒和商陆丝裂原激活分泌免疫球蛋白的不同人类B细胞亚群的频率。
J Exp Med. 1983 Jun 1;157(6):1808-14. doi: 10.1084/jem.157.6.1808.
本文引用的文献
1
Polyclonal activation of human peripheral blood B lymphocytes by formaldehyde-fixed Salmonella paratyphi B. I. Immunoglobulin production without DNA synthesis.甲醛固定的副伤寒乙杆菌对人外周血B淋巴细胞的多克隆激活。I. 无DNA合成的免疫球蛋白产生。
J Exp Med. 1981 Feb 1;153(2):365-74. doi: 10.1084/jem.153.2.365.
2
Cellular mechanisms of restricted immunoglobulin formation in the human neonate.人类新生儿有限免疫球蛋白形成的细胞机制。
Eur J Immunol. 1980 Nov;10(11):888-94. doi: 10.1002/eji.1830101115.
3
Mitotic EBNA-positive lymphocytes in peripheral blood during infectious mononucleosis.传染性单核细胞增多症期间外周血中的有丝分裂EBNA阳性淋巴细胞。
Nature. 1980 Sep 25;287(5780):334-5. doi: 10.1038/287334a0.
4
5
Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line.P3HR-1伯基特淋巴瘤细胞系中的免疫荧光和疱疹病毒样颗粒。
J Virol. 1967 Oct;1(5):1045-51. doi: 10.1128/JVI.1.5.1045-1051.1967.
6
Demonstration of a herpes group virus in cultures of peripheral leukocytes from patients with infectious mononucleosis.在传染性单核细胞增多症患者外周血白细胞培养物中证实有疱疹病毒群病毒。
J Virol. 1968 Jul;2(7):663-9. doi: 10.1128/JVI.2.7.663-669.1968.
7
Comparison of the yield of infectious virus from clones of human and simian lymphoblastoid lines transformed by Epstein-Barr virus.爱泼斯坦 - 巴尔病毒转化的人及猴淋巴母细胞系克隆中传染性病毒产量的比较。
J Exp Med. 1973 Dec 1;138(6):1398-412. doi: 10.1084/jem.138.6.1398.
8
The establishment of lymphoblastoid lines from adult and fetal human lymphoid tissue and its dependence on EBV.从成人和胎儿人类淋巴组织建立淋巴母细胞系及其对EB病毒的依赖性。
Int J Cancer. 1971 Nov 15;8(3):443-50. doi: 10.1002/ijc.2910080312.
9
Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.爱泼斯坦-巴尔病毒（EBV）相关补体结合抗原在产生病毒和不产生病毒的淋巴母细胞系中的细胞定位。
Int J Cancer. 1973 May;11(3):499-520. doi: 10.1002/ijc.2910110302.
10
Various forms of Epstein-Barr virus infection in man: established facts and a general concept.
Lancet. 1973 Oct 13;2(7833):836-9. doi: 10.1016/s0140-6736(73)90874-x.
Zotero 插件