爱泼斯坦-巴尔病毒激活人B淋巴细胞的特征。
Characteristics of Epstein-Barr virus activation of human B lymphocytes.
作者信息
Bird A G, Britton S, Ernberg I, Nilsson K
出版信息
J Exp Med. 1981 Sep 1;154(3):832-9. doi: 10.1084/jem.154.3.832.
Abstract
Epstein-Barr virus (EBV) will infect at least every third cell if exposed in vitro to an extensively purified B cell population from human peripheral blood. About 10% of such infected cells will be driven into immunoglobulin synthesis and secretion, as judged by the indirect protein A plaque assay. The appearance of EB nuclear antigen, de novo DNA synthesis, and immunoglobulin secretion are linked phenomena accompanying infection as judged by viral dilution experiments, which yield kinetics of a one-hit order. Induction of immunoglobulin secretion in B cells by EBV requires de novo synthesis of DNA, and consequently, nontransforming EBV (P3HR1) will not induce immunoglobulin secretion and will also specifically block such induction from subsequently added EBV. The termination of immunoglobulin induction by EBV in short-term cultures appears to be T cell dependent.
摘要
如果在体外将爱泼斯坦-巴尔病毒（EBV）暴露于来自人外周血的高度纯化的B细胞群体，它将至少感染每三个细胞中的一个。通过间接蛋白A空斑试验判断，约10%的此类感染细胞将被驱动进行免疫球蛋白的合成和分泌。通过病毒稀释实验判断，EB核抗原的出现、DNA的从头合成和免疫球蛋白的分泌是伴随感染的相关现象，这些实验产生了一级动力学。EBV诱导B细胞分泌免疫球蛋白需要DNA的从头合成，因此，非转化性EBV（P3HR1）不会诱导免疫球蛋白分泌，并且还会特异性阻断随后添加的EBV的这种诱导作用。在短期培养中，EBV诱导免疫球蛋白的终止似乎依赖于T细胞。
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