Nonaka Kenichi, Todaka Nemuri, Ōmura Satoshi, Masuma Rokuro
1] Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan [2] Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
Kyokuto Pharmaceutical Industrial Co., Ltd., Tokyo, Japan.
J Antibiot (Tokyo). 2014 Nov;67(11):755-61. doi: 10.1038/ja.2014.65. Epub 2014 May 21.
This is the first report describing the improved isolation of common filamentous fungi via a method combining cellulose plate and agar plate system. A cellulose plate is a porous plate made of nanofibrous crystaline cellulose. Isolating fungi from soils using these types of media separately resulted in the number of fungal colonies appearing on cellulose plates being lower than that on agar plates. However, the number of actual fungal species isolated using cellulose plates alone was more or less the same as that found using agar plates. Significantly, the diversity of isolates using a combination of the two media was greater than using each media individually. As a result, numerous new or rare fungal species with potential, including previously proposed new species, were isolated successfully in this way. All fungal colonies, including the Penicillium species, that appeared on the cellulose plate penetrated in potato dextrose were either white or yellow. Cultivation on cellulose plates with added copper ion overcomes the change in coloration, the colonies appearing as they do following cultivation on potato dextrose agar.
这是第一篇描述通过纤维素平板和琼脂平板系统相结合的方法改进常见丝状真菌分离的报告。纤维素平板是由纳米纤维结晶纤维素制成的多孔平板。单独使用这些类型的培养基从土壤中分离真菌，导致纤维素平板上出现的真菌菌落数量低于琼脂平板上的数量。然而，仅使用纤维素平板分离出的实际真菌种类数量与使用琼脂平板发现的数量大致相同。值得注意的是，使用两种培养基组合分离出的菌株多样性大于单独使用每种培养基。结果，通过这种方式成功分离出了许多具有潜力的新的或罕见的真菌种类，包括先前提出的新物种。在马铃薯葡萄糖培养基中生长的纤维素平板上出现的所有真菌菌落，包括青霉属菌种，要么是白色要么是黄色。在添加了铜离子的纤维素平板上培养可克服颜色变化，菌落呈现出在马铃薯葡萄糖琼脂上培养后的样子。
