Hamzehee Sara, Gharaghani Maral, Borsi Seyed Hamid, Jafarian Hadis, Jalaee Gholam Ali, Kardooni Maryam, Zarei-Mahmoudabadi Ali
Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Medicinal Plants Research Center, Yasouj University of Medical Sciences, Yasouj, Iran.
BMC Microbiol. 2025 Sep 1;25(1):571. doi: 10.1186/s12866-025-04323-7.
Aspergillus section Nigri is widely distributed in decaying plant materials in our environment. Although most of these species are known for food spoilage and some have industrial applications, some of these species, including Aspergillus tubingensis, A. welwitschiae, and A. niger, are etiologic agents of human and animal aspergillosis. Moreover, the frequency of each species in different environments is correlated with meteorological conditions. The present study aimed to investigate the frequency of Aspergillus section Nigri in clinical samples and environmental sources in several Iranian provinces.
A total of 629 samples, including 261 clinical materials and 368 environmental materials, were collected from seven Iranian provinces. The Aspergillus section Nigri was initially screened on the basis of colony morphology and then subjected to sequencing via the calmodulin (CaM) gene. Maximum likelihood phylogenies were constructed via MEGA 11 software.
Of the 192 Aspergillus section Nigri isolates, 41.15% were identified as A. tubingensis, followed by A. welwitschiae (35.94%), A. niger (sensu stricto) (17.71%), A. neoniger (1.56%), A. piperis (1.56%), A. aculeatus (1.04%), and A. luchuensis (1.04%). The most prevalent species were A. tubingensis and A. welwitschiae from clinical and environmental samples, respectively. In addition, the frequency of these species varied across Iranian provinces.
This study determined the presence and frequency, distribution, and phylogenetic relationships of eight black aspergilli, A. tubingensis, A. welwitschiae, A. niger, A. neoniger, A. piperis, A. aculeatus, and A. luchuensis, associated with clinical and environmental samples from several Iranian provinces. In terms of the source of isolation, A. tubingensis was most common in the both environmental and clinical samples, whereas A. welwitschiae was most common in the clinical samples.
黑曲霉组在我们环境中的腐烂植物材料中广泛分布。尽管这些物种大多以造成食物腐败而闻名，有些还具有工业应用，但其中一些物种，包括管囊曲霉、韦氏曲霉和黑曲霉，是人和动物曲霉病的病原体。此外，不同环境中每个物种的出现频率与气象条件相关。本研究旨在调查伊朗几个省份临床样本和环境来源中黑曲霉组的出现频率。
从伊朗七个省份共收集了629份样本，包括261份临床材料和368份环境材料。最初根据菌落形态对黑曲霉组进行筛选，然后通过钙调蛋白（CaM）基因进行测序。使用MEGA 11软件构建最大似然系统发育树。
在192株黑曲霉组分离株中，41.15%被鉴定为管囊曲霉，其次是韦氏曲霉（35.94%）、狭义黑曲霉（17.71%）、新黑曲霉（1.56%）、胡椒曲霉（1.56%）、棘孢曲霉（1.04%）和鲁氏曲霉（1.04%）。临床样本和环境样本中最常见的物种分别是管囊曲霉和韦氏曲霉。此外，这些物种在伊朗各省的出现频率有所不同。
本研究确定了与伊朗几个省份临床和环境样本相关的八种黑曲霉，即管囊曲霉、韦氏曲霉、黑曲霉、新黑曲霉、胡椒曲霉、棘孢曲霉和鲁氏曲霉的存在、频率、分布及系统发育关系。就分离来源而言，管囊曲霉在环境样本和临床样本中最为常见，而韦氏曲霉在临床样本中最为常见。
