Salehinejad Jahanshah, Sharifi Nourieh, Amirchaghmaghi Maryam, Ghazi Narges, Shakeri Mohammad Taghi, Ghazi Ala
Dental Research Center, Department of Oral and Maxillofacial Pathology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Ann Diagn Pathol. 2014 Aug;18(4):210-3. doi: 10.1016/j.anndiagpath.2014.03.009. Epub 2014 Apr 13.
Epithelial carcinogenesis is a multistep process. Specific genetic events lead to malignant transformation of oral epithelium. Oral squamous cell carcinoma (OSCC) may be preceded by potentially malignant lesions such as oral lichen planus (OLP). The p16 protein functions as a negative regulator of the cell cycle progression. Altered pattern of p16 serves as a biomarker for oral mucosal dysplasia and malignant growth. The purpose of this study was to evaluate p16 expression in OSCC and OLP to determine whether it can be a useful marker for early detection of carcinogenesis. We examined p16 expression in 45 OSCCs (15 grade I, 15 grade II, and 15 grade III), 15 OLPs without dysplasia, and 8 normal mucosal specimens with immunohistochemistry. p16 was interpreted as positive if more than 70% of tumor cells showed brown nuclear and cytoplasmic staining. All of the OSCC and control group samples showed negative immunoreactivity, whereas 26.7% of OLP samples were positive for p16. Our findings suggest that p16 expression could not be used as a helpful marker for detection of development toward malignancy in OLP samples.
上皮细胞癌变是一个多步骤过程。特定的基因事件会导致口腔上皮发生恶性转化。口腔鳞状细胞癌（OSCC）可能由潜在恶性病变如口腔扁平苔藓（OLP）发展而来。p16蛋白作为细胞周期进程的负调节因子发挥作用。p16表达模式的改变可作为口腔黏膜发育异常和恶性生长的生物标志物。本研究的目的是评估p16在OSCC和OLP中的表达情况，以确定其是否可作为癌变早期检测的有用标志物。我们采用免疫组化方法检测了45例OSCC（15例I级、15例II级和15例III级）、15例无发育异常的OLP以及8例正常黏膜标本中的p16表达。如果超过70%的肿瘤细胞呈现棕色核染色和胞质染色，则p16被判定为阳性。所有OSCC和对照组样本均显示免疫反应阴性，而26.7%的OLP样本p16呈阳性。我们的研究结果表明，p16表达不能作为检测OLP样本向恶性发展的有用标志物。
