Lassalle Sandra, Hofman Véronique, Ilie Marius, Butori Catherine, Bonnetaud Christelle, Gaziello Marie Clotilde, Selva Eric, Gavric-Tanga Virginie, Guevara Nicolas, Castillo Laurent, Santini José, Chabannon Christian, Hofman Paul
1 Biobank Unit, Pasteur Hospital , Nice, France .
Biopreserv Biobank. 2011 Mar;9(1):9-19. doi: 10.1089/bio.2010.0024. Epub 2011 Mar 22.
In the last few years, conditions for setting up a human biobank in France have been upgraded by taking into account (1) the new laws and regulations that integrate the ethical and societal dimension of biobanking and delineate the risks for patients associated with the procurement of human cells and tissues, (2) the increasing request by scientists for human samples with proven biological quality and sophisticated sets of annotations, including information produced through the evergrowing use of molecular biology in pathology, and (3) establishment of procedures concerning the safety of the personnel working with biological products. For this purpose, health authorities and national research institutes in France have provided significant support for the set up of biobanks. The present work was conducted to describe how we set up a biobank targeting diseases of a specific organ (thyroid gland), with the aim of rapidly developing translational research projects. The prospective experience of a single institution (Pasteur Hospital, Nice, France) over a 6-year period (2004-2009) is presented from the practical point of view of a surgical pathology laboratory. We describe different procedures required to obtain high-quality thyroid biological resources and clinical annotations. The procedures were established for the management of biological products obtained from 1454 patients who underwent thyroid surgery. The preanalytical steps leading to the storage of frozen specimens were carried out in parallel with diagnostic procedures. As the number of international networks for research programs using biological products is steadily increasing, it is crucial to harmonize the procedures used by biobanks. In this regard, the described thyroid biobank has been set up using criteria established by the French National Cancer Institute (Institut National du Cancer) to guarantee the quality of different collections stored in biobanks.
在过去几年中，法国建立人类生物样本库的条件得到了提升，这是考虑到以下几点：（1）新的法律法规纳入了生物样本库的伦理和社会层面，并明确了与人类细胞和组织采集相关的患者风险；（2）科学家对具有可靠生物学质量和复杂注释集的人类样本的需求不断增加，包括通过病理学中分子生物学的日益广泛应用所产生的信息；（3）制定了有关处理生物制品工作人员安全的程序。为此，法国的卫生当局和国家研究机构为生物样本库的建立提供了大力支持。本研究旨在描述我们如何建立一个针对特定器官（甲状腺）疾病的生物样本库，以期迅速开展转化研究项目。本文从外科病理实验室的实际角度，介绍了法国尼斯巴斯德医院这一单一机构在6年期间（2004 - 2009年）的前瞻性经验。我们描述了获取高质量甲状腺生物资源和临床注释所需的不同程序。这些程序是为管理从1454例接受甲状腺手术的患者身上获取的生物制品而制定的。导致冷冻标本储存的分析前步骤与诊断程序并行进行。由于使用生物制品的国际研究项目网络数量在稳步增加，统一生物样本库使用的程序至关重要。在这方面，所描述的甲状腺生物样本库是按照法国国家癌症研究所制定的标准建立的，以确保生物样本库中储存的不同样本的质量。
