Lenn N J, Seeley P J, Field P M, Raisman G
Division of Pediatric Neurology, University of Virginia Medical Center, Charlottesville 22908.
J Neural Transplant. 1989;1(2):57-62. doi: 10.1155/NP.1989.57.
The effects of donor age and site of placement on the survival of fetal medial habenula (MH) transplants into adult rats hosts were examined. The innervation of the interpeduncular nucleus (IPN) in such cases was also examined. Explants of MH consisting of the medial-dorsal lip of the third ventricle were held in vitro for 1-2 days. Colloidal gold conjugated to wheat germ agglutinin was added for the last 18 hours to label the cells. Four of 16 cases with E19 derived transplants contained donor neurons. Markedly larger transplants were present in 95% of 20 cases with E16 derived transplants. Sites in the ventral midbrain were successful, while limited or no survival occurred at sites more remote from IPN. Retrograde labeling of transplant neurons was present in each case studied with HRP injection into host IPN. Colloidal gold-labeled macrophages, some oriented capillaries and GFAP-positive processes marked the donor-host interface. In EM the interface was evident only by the difference in tissue elements in the transplant versus host. Numerous synapses of Gray types I and II were present in the transplant. Excellent survival of MH neurons, donor/host interfaces, innervation of IPN by the transplant and fine structure in and around the transplants, all suggest that such preparations are suitable for further experimental analysis of the habenulo-interpeduncular system.
研究了供体年龄和移植部位对胎鼠内侧缰核（MH）移植到成年大鼠宿主体内存活的影响。同时也研究了此类情况下脚间核（IPN）的神经支配情况。由第三脑室内侧背唇组成的MH外植体在体外培养1 - 2天。在最后18小时添加与小麦胚芽凝集素偶联的胶体金来标记细胞。16例E19来源移植病例中有4例含有供体神经元。20例E16来源移植病例中有95%出现明显更大的移植体。腹侧中脑的部位移植成功，而距离IPN较远的部位移植体存活有限或未存活。在每例向宿主IPN注射HRP进行研究的病例中均出现移植神经元的逆行标记。胶体金标记的巨噬细胞、一些定向的毛细血管和GFAP阳性突起标记了供体 - 宿主界面。在电子显微镜下，界面仅通过移植组织与宿主组织成分的差异得以显现。移植体中存在大量I型和II型Gray突触。MH神经元的良好存活、供体/宿主界面、移植体对IPN的神经支配以及移植体及其周围的精细结构，均表明此类制备物适合用于对缰核 - 脚间系统进行进一步的实验分析。
