Haun F, Eckenrode T C, Murray M
Department of Anatomy and Neurobiology, Medical College of Pennsylvania, Philadelphia 19129.
J Neurosci. 1992 Aug;12(8):3282-90. doi: 10.1523/JNEUROSCI.12-08-03282.1992.
The preceding companion study (Eckenrode et al., 1992) showed that cell suspension transplants of fetal habenula cells placed near the interpeduncular nucleus (IPN) following lesions of the fasciculus retroflexus (FR) restore the normal pattern of substance P (SP) staining in habenular target subnuclei of the IPN in both perinatal and adult hosts, and restore ChAT staining in the IPN of perinatal hosts. Similarly placed transplants of fetal thalamus cells only restore ChAT staining in the IPN of adult hosts. In this study, we examined the functional significance of these restored staining patterns. We used a behavioral measure of the integrity of REM-stage and non-REM-stage sleep, the "flower pot" test, and assayed (1) normal adult rats, (2) FR-lesioned control animals (neonatal or adult operates), (3) animals receiving FR lesions and transplants of fetal habenula cells (perinatal or adult hosts), and (4) animals receiving FR lesions and transplants of fetal thalamus cells (adult hosts). FR lesions decrease markedly the muscle atonia component of REM sleep and reduce duration of sleep episodes. Transplants that restore SP staining in the IPN (habenular transplants into either perinatal or adult lesion hosts) restore normal frequency of REM atonia; transplants that restore ChAT staining (habenular transplants into perinatal hosts or thalamic transplants into adult hosts) restore normal duration of sleep episodes. The number of SP-immunoreactive cells in the transplants predicts recovery of REM atonia, and the number of ChAT cells in habenular (but not thalamic) transplants predicts restoration of sleep duration.(ABSTRACT TRUNCATED AT 250 WORDS)
之前的配套研究（埃肯罗德等人，1992年）表明，在扣带回束（FR）损伤后，将胎儿缰核细胞悬液移植到脚间核（IPN）附近，可使围产期和成年宿主IPN缰核靶亚核中P物质（SP）染色恢复正常模式，并使围产期宿主IPN中的胆碱乙酰转移酶（ChAT）染色恢复正常。同样位置的胎儿丘脑细胞移植仅能使成年宿主IPN中的ChAT染色恢复正常。在本研究中，我们检验了这些恢复的染色模式的功能意义。我们使用了一种衡量快速眼动睡眠期和非快速眼动睡眠期睡眠完整性的行为测试——“花盆”测试，并对以下几组动物进行了分析：（1）正常成年大鼠；（2）FR损伤的对照动物（新生或成年手术组）；（3）接受FR损伤并移植胎儿缰核细胞的动物（围产期或成年宿主）；（4）接受FR损伤并移植胎儿丘脑细胞的动物（成年宿主）。FR损伤显著降低了快速眼动睡眠期的肌肉弛缓成分，并缩短了睡眠时长。能使IPN中SP染色恢复的移植（将缰核移植到围产期或成年损伤宿主中）可使快速眼动睡眠期肌肉弛缓的频率恢复正常；能使ChAT染色恢复的移植（将缰核移植到围产期宿主中或将丘脑移植到成年宿主中）可使睡眠时长恢复正常。移植中SP免疫反应性细胞的数量可预测快速眼动睡眠期肌肉弛缓的恢复情况，而缰核（而非丘脑）移植中ChAT细胞的数量可预测睡眠时长的恢复情况。（摘要截断于250词）
