Eckenrode T C, Murray M, Haun F
Department of Anatomy and Neurobiology, Medical College of Pennsylvania, Philadelphia 19129.
J Neurosci. 1992 Aug;12(8):3272-81. doi: 10.1523/JNEUROSCI.12-08-03272.1992.
Innervation of specific peptidergic and cholinergic compartments of the interpeduncular nucleus (IPN) was investigated using embryonic cell suspension transplants immunoreactive for substance P (SP) and ChAT. In both neonatal and adult host rats, the IPN was first denervated of its normal SP and cholinergic input from the medial habenula by bilateral lesions of the fasciculi retroflexi (FR). In adult hosts, transplants of embryonic habenular cells placed near the denervated IPN mediated a return of the normal pattern of SP staining restricted to habenula-target subnuclei, plus an increase in staining intensity of SP cells intrinsic to the IPN. There was no recovery of ChAT staining. A similar pattern of SP staining resulted following habenular transplants into neonatal hosts, but in addition there was a partial recovery of normal ChAT staining in cholinergic subnuclei and anomalous ChAT staining in normally peptidergic subnuclei. Control transplants of embryonic thalamus cells placed into adult hosts produced a surprising pattern of ChAT staining in the IPN identical to that seen with habenula transplants placed into neonatal hosts; the adult IPN was thus able to support reinnervation mediated by an aberrant cholinergic source while being refractory to its normal habenular cholinergic afferents. This pattern of results implies regulation by the IPN of habenular SP and cholinergic innervation, and some interaction between the maturing normal cholinergic afferents and their targets that is missing when these afferent sources are abnormal.
利用对P物质（SP）和胆碱乙酰转移酶（ChAT）呈免疫反应的胚胎细胞悬液移植，研究了脚间核（IPN）特定肽能和胆碱能区室的神经支配情况。在新生和成年宿主大鼠中，首先通过双侧缰核后束（FR）损伤，使IPN失去来自内侧缰核的正常SP和胆碱能输入。在成年宿主中，将胚胎缰核细胞移植到去神经支配的IPN附近，介导了正常SP染色模式的恢复，该模式仅限于缰核靶向亚核，同时IPN内源性SP细胞的染色强度增加。ChAT染色没有恢复。将缰核移植到新生宿主后也出现了类似的SP染色模式，但此外，胆碱能亚核中正常ChAT染色部分恢复，而在正常肽能亚核中出现异常ChAT染色。将胚胎丘脑细胞移植到成年宿主的对照实验中，在IPN中产生了一种令人惊讶的ChAT染色模式，与将缰核移植到新生宿主中所见的模式相同；因此，成年IPN能够支持由异常胆碱能来源介导的再支配，而对其正常的缰核胆碱能传入神经却具有抗性。这种结果模式意味着IPN对缰核SP和胆碱能神经支配具有调节作用，并且在这些传入神经来源异常时，成熟的正常胆碱能传入神经与其靶标之间存在一些缺失的相互作用。
