Zhu Jia-Ying, Wu Guo-Xing, Ze Sang-Zi, Stanley David W, Yang Bin
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China.
College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
J Insect Physiol. 2014 Jul;66:37-44. doi: 10.1016/j.jinsphys.2014.05.011. Epub 2014 May 19.
Ectoparasitoid wasps deposit their eggs onto the surface and inject venom into their hosts. Venoms are chemically complex and they exert substantial impact on hosts, including permanent or temporary paralysis and developmental arrest. These visible venom effects are due to changes in expression of genes encoding physiologically relevant proteins. While the influence of parasitization on gene expression in several lepidopterans has been reported, the molecular details of parasitoid/beetle relationships remain mostly unknown. This shortcoming led us to pose the hypothesis that envenomation by the ectoparasitic ant-like bethylid wasp Scleroderma guani leads to changes in protein expression in the yellow mealworm beetle Tenebrio molitor. We tested our hypothesis by comparing the proteomes of non-parasitized and parasitized host pupae using iTRAQ-based proteomics. We identified 41 proteins that were differentially expressed (32↑- and 9↓-regulated) in parasitized pupae. We assigned these proteins to functional categories, including immunity, stress and detoxification, energy metabolism, development, cytoskeleton, signaling and others. We recorded parallel changes in mRNA levels and protein abundance in 14 selected proteins following parasitization. Our findings support our hypothesis by documenting changes in protein expression in parasitized hosts.
外寄生蜂将卵产在宿主表面，并向宿主体内注入毒液。毒液的化学成分复杂，会对宿主产生重大影响，包括永久性或暂时性麻痹以及发育停滞。这些明显的毒液效应是由于编码生理相关蛋白质的基因表达发生了变化。虽然已有报道称寄生会对几种鳞翅目昆虫的基因表达产生影响，但寄生蜂与甲虫关系的分子细节大多仍不清楚。这一缺陷促使我们提出假设，即外寄生的蚁形甲腹茧蜂中华硬皮肿腿蜂注入的毒液会导致黄粉虫蛹的蛋白质表达发生变化。我们通过基于iTRAQ的蛋白质组学比较未寄生和寄生宿主蛹的蛋白质组来检验我们的假设。我们鉴定出41种在寄生蛹中差异表达的蛋白质（32种上调和9种下调）。我们将这些蛋白质归类到功能类别中，包括免疫、应激和解毒、能量代谢、发育、细胞骨架、信号传导等。我们记录了寄生后14种选定蛋白质的mRNA水平和蛋白质丰度的平行变化。我们的研究结果通过记录寄生宿主中蛋白质表达的变化支持了我们的假设。
