二肽基肽酶 IV 的分子特征和功能分析来自外寄生蜂的毒液。
Molecular Characterization and Functional Analysis of the Dipeptidyl Peptidase IV from Venom of the Ectoparasitoid .
机构信息
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, China.
Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, China.
出版信息
Toxins (Basel). 2023 Apr 27;15(5):311. doi: 10.3390/toxins15050311.
Dipeptidyl peptidase IV (DPPIV) is a proline-specific serine peptidase that remains poorly investigated in terms of venom composition. Here, we describe the molecular characteristics and possible functions of DPPIV as a major venom component of the ant-like bethylid ectoparasitoid, , named SgVnDPPIV. The SgVnDPPIV gene was cloned, which encodes a protein with the conserved catalytic triads and substrate binding sites of mammalian DPPIV. This venom gene is highly expressed in the venom apparatus. Recombinant SgVnDPPIV, produced in Sf9 cells using the baculovirus expression system, has high enzymatic activity, which can be efficiently inhibited by vildagliptin and sitagliptin. Functional analysis revealed that SgVnDPPIV affects genes related to detoxification, lipid synthesis and metabolism, response to stimuli, and ion exchange in pupae of , an envenomated host of . The present work contributes towards understanding the role of venom DPPIV involved in the interaction between parasitoid wasp and its host.
二肽基肽酶 IV（DPPIV）是一种脯氨酸特异性丝氨酸肽酶，其毒液组成方面的研究仍很不完善。在这里，我们描述了作为类似蚂蚁的双翅目外寄生蜂 ，即 SgVnDPPIV 的主要毒液成分的 DPPIV 的分子特征和可能的功能。克隆了 SgVnDPPIV 基因，该基因编码一种具有哺乳动物 DPPIV 的保守催化三联体和底物结合位点的蛋白质。这种毒液基因在毒液器官中高度表达。使用杆状病毒表达系统在 Sf9 细胞中产生的重组 SgVnDPPIV 具有很高的酶活性，可被维达列汀和西他列汀有效抑制。功能分析表明，SgVnDPPIV 影响与解毒、脂质合成和代谢、对刺激的反应以及 ，一种被毒液侵染的宿主 ，蛹中的离子交换有关的基因。本研究有助于了解参与寄生蜂与其宿主相互作用的毒液 DPPIV 的作用。
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