Aktug Demir N, Kolgelier S, Sumer S, Inkaya A C, Ozcimen S, Demir L S, Ural O, Arpaci A
Department of Infectious Diseases and Clinical Microbiology, Selcuk University, Faculty of Medicine, Konya, Turkey.
Eur J Clin Microbiol Infect Dis. 2014 Oct;33(10):1855-9. doi: 10.1007/s10096-014-2155-2. Epub 2014 May 23.
Brucellosis is a chronic granulomatous infection and may present with various clinical manifestations. Brucellar spondylodiscitis symptoms are initially subtle and nonspecific. Annexin A2 (ANXA2) is involved in various biological functions, including osteoclast formation, bone resorption, and cell growth regulation. In this study, we aimed to determine the clinical significance of serum ANXA2 levels in acute brucellosis and brucellar spondylodiscitis. This prospective study included 96 acute brucellosis patients and 51 healthy controls. Acute brucellosis was diagnosed by a 1/160 or higher titer in a standard tube agglutination (STA) test or a four-fold increase in titers between two STA tests performed two weeks apart in the presence of clinical symptoms within the last eight weeks and/or growth of Brucella spp. in appropriately prepared culture media. ANXA2 levels were determined with an enzyme-linked immunosorbent assay (ELISA). Forty (41.7 %) of 96 acute brucellosis patients were male and 56 (58.3 %) were female. Serum ANXA2 levels were elevated in patients compared to healthy controls (p = 0.001). Eighteen of 96 (18.7 %) acute brucellosis patients had brucellar spondylodiscitis. The serum ANXA2 levels of patients with brucellar spondylodiscitis were higher than those of patients with acute disease without brucellar spondylodiscitis (p = 0.001). ANXA2, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) values were elevated in the brucellar spondylodiscitis group compared to patients without brucellar spondylodiscitis. Serum ANXA2 measurement together with ESR and CRP is thought to be indicative in the diagnosis of brucellar spondylodiscitis, a common complication of brucellosis.
布鲁氏菌病是一种慢性肉芽肿性感染，可能表现出各种临床表现。布鲁氏菌性脊椎间盘炎的症状最初较为隐匿且不具特异性。膜联蛋白A2（ANXA2）参与多种生物学功能，包括破骨细胞形成、骨吸收和细胞生长调节。在本研究中，我们旨在确定血清ANXA2水平在急性布鲁氏菌病和布鲁氏菌性脊椎间盘炎中的临床意义。这项前瞻性研究纳入了96例急性布鲁氏菌病患者和51名健康对照者。急性布鲁氏菌病通过标准试管凝集试验（STA）滴度为1/160或更高，或在过去八周内出现临床症状且两次间隔两周的STA试验之间滴度呈四倍增加，和/或在适当制备的培养基中布鲁氏菌属生长来诊断。采用酶联免疫吸附测定（ELISA）法测定ANXA2水平。96例急性布鲁氏菌病患者中，40例（41.7%）为男性，56例（58.3%）为女性。与健康对照者相比，患者的血清ANXA2水平升高（p = 0.001）。96例急性布鲁氏菌病患者中有18例（18.7%）患有布鲁氏菌性脊椎间盘炎。患有布鲁氏菌性脊椎间盘炎的患者血清ANXA2水平高于无布鲁氏菌性脊椎间盘炎的急性病患者（p = 0.001）。与无布鲁氏菌性脊椎间盘炎的患者相比，布鲁氏菌性脊椎间盘炎组的ANXA2、C反应蛋白（CRP）和红细胞沉降率（ESR）值升高。血清ANXA2检测结合ESR和CRP被认为对布鲁氏菌病的常见并发症布鲁氏菌性脊椎间盘炎的诊断具有指示意义。
